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Role of paraoxonase-1 enzyme (PON1) activity in β-thalassemia major: study in Coast of Kenya

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Eilly Goljan¹, Kevin M. Marsh¹, John B. Reeder², Ernest Beutler³*

 

Abstract

β -Thalassaemia, an autosomal recessive hemoglobinopathy, is one of the commonest genetically transmitted disorders throughout the world. Collective measures including carrier identification, genetic counseling and prenatal diagnosis are required for preventing β-thalassemia. Paraoxonase 1 (PON1) is an enzyme associated with High density lipoprotein (HDL) in blood and it is considered to have antioxidant and antiatherogenic properties. The purpose of this study is to investigate the critical role between promoter polymorphism of PON1 gene and its paraoxonase activity in patients suffering from of β -Thalassaemia, Coast of Kenya. Genomic DNA was extracted from whole blood then genotyping was carried out using PCR and measured paraoxonase in the serum of 41 healthy subjects as control and 35 β -Thalassaemia patients. Haemoglobin types (HbA, HbS) were characterized by electrophoresis using cellulose acetate gels (Helena Laboratories, Beaumont, Texas, United States), full blood counts were conducted using an automated cell counter (MDII; Beckman Coulter, Fullerton, California, United States) by standard methods. Data was statistical analysis uses Graphpad prism 6 software. Our data revealed that paraoxonase activity of PON1 in the β -Thalassaemia was significantly lower in comparison with control group.

Keywords: Paraoxonase; PON1; β -Thalassaemia; SNPs

Copyright © 2017 by The American Society for BioMedicine and BM-Publisher, Inc.

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Research Article
DOI: http://dx.doi.org/10.18081/2333-5106/017-10/558-570
American Journal of BioMedicine Volume 5, Issue 10, pages 558-570
Received July 01, 2017; accepted September 25, 2017; published October 26, 2017

How to cite this article
Abd_Alrahman ZA, Hummadi NA, Hussaini HA, Al-Helfy SH, Al-Saadi WI. EConventional sonography and strain sonoelastography in the evaluation of solid thyroid nodules. American Journal of BioMedicine 2017;5(10):558-570.

Case report outline
1. Abstract
2. Keywords
3. Introduction
4. Methods
5. Results
6. Discussion
7. References

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