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Adhesion molecule expression trigger immune-mediated pathology in lupus-nephritis

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American Journal of BioMedicine  Volume 1, Issue 2, pages 49–57, December 2013

doi: 10.18081/ajbm/2333-5106-013-12/49-57


Jens Boldron, Simon Caltabiano, Steven D. Debono, Robert Thompson, Malcolm Scammells, Robert Westover, Ye Wu, Philip Frugier

Abstract

Systemic lupus erythematosus (SLE) is an autoimmune disease that affects many organs including the joints, kidneys and brain. Lupus nephritis is a potentially devastating complication of SLE. Immune cells, cytokines, and epigenetic factors have all been recently implicated in lupus nephritis pathogenesis. We have hypothesized that there Adhesion molecule expression trigger immune-mediated pathology in lupus-nephritis. We used female MRL/lpr, MRL/mpJ (develops lupus-like disease at 10-14 months of age) and C3H/HeJ mice. Using immunofluorescent antibody binding assays and confocal laser imaging, we show that expression of ICAM-1 and VCAM-1 is elevated in MRL/lpr mice brains at 3-6 months of age as compared to age-matched controls. These results suggest a possible mechanism for leukocyte entry into the brains of autoimmune mice that in turn suggest immune-mediated pathology in CNS-lupus and may enable a paradigm shift in the treatment of this complex disease.

Keywords: Systemic lupus erythematosus; Adhesion molecule expression; cytokines; ICAM-1; VCAM-1


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