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Etanerecept ameliorate inflammatory responses and apoptosis induces by myocardial ischemia/reperfusion in male mice

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American Journal of BioMedicine  Volume 2, Issue 6, pages 732-744, June 2014


Najah R. Hadi, Fadhil G. Al-Amran, Maitham G Yousif,  Saif M. Hassan

 Abstract

Ischemia/reperfusion of cardiac tissues may lead to a prominent damage of the cardiomyocyte through either necrosis or apoptosis. In this study, we investigated the effects of Etanercept in myocardial ischemia/reperfusion injury. Acute myocardial injury was induced by ligation of the left anterior descending coronary artery (LAD) for 30 minutes followed by 120 minutes reperfusion in white albino adult male. At the end of reperfusion, cardiac tissue TNF-α, IL-1β, IL-6, caspase-3 and Bcl-2, as well as plasma cardiac troponin I (cTn-I) were measured. It has been found that Etanercept treated group showed significant reduction in TNF-α, IL-1β, IL-6, caspase-3, Bcl-2 and cTn-I with respect to the control groups (P˂0.01). Histopathology study revealed that the treatment with Etanercept significantly improved cardiac injury as compared with control groups (P˂0.05). Further, the total severity scores showed 16.7 % of the group had no damage, while 50% had mild cardiac injury and only 33.3% with moderate cardiac injury in Etanercept treated group. We demonstrated that Etanercept reduces inflammatory reaction, cardiomyocyte apoptosis and myocardial damage induced by ischemia/reperfusion injury.

Keywords: Etanercept; Caspase-3; Ischemia/reperfusion; LAD; Myocardial injury


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