Advertisement

Notch-3 promote angiogenesis and proliferation of bladder cancer cells through the PI3K/Akt pathway

[Limited Access]               Full Text-PDF

American Journal of BioMedicine  Volume 2, Issue 6, pages 724-731, June 2014


Joan V. Draucker; Irene Talarico; Ling Wu; David B. Mulier; Xian Chen; Celeste B. Determan; Jean Friese; Ling Yao

Abstract

Transitional cell carcinoma (TCC) of the bladder is the most common form of bladder cancer and is manifested in two distinct forms with different clinical and biological behaviors. Approximately 70% of patients present with non-muscle invasive tumors, while the remaining 30% present with muscle-invasive tumors. Despite good prognosis for patients with superficial disease, recurrence is common and is associated with development of muscle-invasive disease. Seventy seven tissue specimens from bladder cancer patient [32- noninvasive and 45- muscle invasive] and forty specimens from patients with benign prostatic hypertrophy as control were enrolled. The specimen’s tissue was halved into either in 10% neutral buffered formalin for histological process or snap frozen storage in liquid nitrogen. Tissue RNA and protein were examined by qPCR and western blot for Noch-3 and PI3K/Akt pathway respectively. One-Way ANOVA with Bonferroni tests was applied, with significance accepted at P<0.05. Our results highlight critical, overlooked functions for Notch-3 in regulating angiogenesis and proliferation of bladder cancer cells and suggest that Notch-3 inhibitor may be drug target in bladder cancer. Further investigation into the precise mechanism for this protection is warranted.

Keyword: Bladder cancer; Noch-3; PI3K/Akt pathway; Angiogenesis; Proliferation


References

1. Ferlay J, Autier P, Boniol M, Heanue M, Colombet M, et al. Estimates of the cancer incidence and mortality in Europe in 2006. Ann Oncol 2007;18: 581–592. [PubMed]

2. Lopez-Beltran A, Montironi R. Non-invasive urothelial neoplasms: according to the most recent WHO classification. Eur Urol 2004;46: 170–176. [PubMed]

3. Boulalas I, Zaravinos A, Karyotis I, Delakas D, Spandidos DA (2009) Activation of RAS family genes in urothelial carcinoma. J Urol 181: 2312–2319. Boulalas I, Zaravinos A, Karyotis I, Delakas D, Spandidos DA. Activation of RAS family genes in urothelial carcinoma. J Urol 2009;181: 2312–2319. [PubMed]

4. Vallmanya Llena FR, Laborda Rodriguez A, Lloreta Trull J, Cortadellas Angel R, Placer Santos J, et al. [Immunohistochemical expression of p53, p21, p16, and cyclin D1 in superficial bladder cancer. A tissue microarray study]. Actas Urol Esp 2006;30: 754–762. [PubMed]

5. Botteman MF, Pashos CL, Redaelli A, Laskin B, Hauser R. The health economics of bladder cancer – A comprehensive review of the published literature. Pharmacoeconomics 2003;21: 1315–1330. [PubMed]

6. Yu J, Zhu T, Wang Z, Zhang H, Qian Z, et al. A novel set of DNA methylation markers in urine sediments for sensitive/specific detection of bladder cancer. Clin Cancer Res 2007;13: 7296–7304. [PubMed]

7. Marsit CJ, Christensen BC, Houseman EA, Karagas MR, Wrensch MR, et al. Epigenetic profiling reveals etiologically distinct patterns of DNA methylation in head and neck squamous cell carcinoma. Carcinogenesis 2009;30: 416–422. [PubMed]

8. Khin SS, Kitazawa R, Win N, Aye TT, Mori K, et al. BAMBI gene is epigenetically silenced in subset of high-grade bladder cancer. Int J Cancer 2009;125: 328–338. [PubMed]

9. Hunkapiller NM, Gasperowicz M, Kapidzic M, Plaks V, Maltepe E, et al. A role for Notch signaling in trophoblast endovascular invasion and in the pathogenesis of pre-eclampsia. Development 2011;138: 2987–2998. [PubMed]

10. Groot AJ, Habets R, Yahyanejad S, Hodin CM,  Reiss K,  Saftig P, et al.Regulated Proteolysis of NOTCH2 and NOTCH3 Receptors by ADAM10 and Presenilins Mol. Cell. Biol 2014: 28222832. [Abstract/Full-Text]

11. De Salvo M, Raimondi L, Vella S, Adesso L, Ciarapica R, Verginelli F, Pannuti A, Citti A, et al. Hyper-activation of notch3 amplifies the proliferative potential of rhabdomyosarcoma cells. PLoS One 2014 May 5;9(5):e96238. [PubMed]

12. Artavanis-Tsakonas S, Rand MD, Lake RJ. Notch signaling: cell fate control and signal integration in development. Science 1999;284: 770–776. [PubMed]

13. Doshi WB, Thury PG, Chen R, Fishbein JA,  Foley MK. Synergistic interaction between AKT and Notch-1 signaling in cervical cancer: a critical role in tumor cell migration and invasion. American journal of BioMedicine 2014;2:200-211. [Abstract/Full-Text]

14. Yousif NG, Al-Matwari M. Overexpression of Notch-1 induced tamoxifen resistance through down regulation of ESR1 in positive estrogen receptor breast cancer. JOURNAL OF CLINICAL ONCOLOGY 2012;30(15). [Google scholar]

15. Conboy IM, Conboy MJ, Smythe GM, Rando TA Notch-mediated restoration of regenerative potential to aged muscle. Science 2003;302: 1575–1577. [PubMed]

16. Gagan J, Dey BK, Layer R, Yan Z, Dutta A.  Notch3 and Mef2c proteins are mutually antagonistic via Mkp1 protein and miR-1/206 microRNAs in differentiating myoblasts. J Biol Chem 2012;287: 40360–40370. [PubMed]

17. Dahlqvist C, Blokzijl A, Chapman G, Falk A, Dannaeus K, et al. Functional Notch signaling is required for BMP4-induced inhibition of myogenic differentiation. Development 2003;130: 6089–6099. [Abstract/Full-Text]

18. Berezovska O, Jack C, McLean P, Aster JC, Hicks C, Xia W, Wolfe MS, Weinmaster G, Selkoe DJ, Hyman BT. Rapid Notch1 nuclear translocation after ligand binding depends on presenilin-associated gamma-secretase activity. Ann. NY Acad. Sci 2000;920:223 -226. [PubMed]

19. Morrison SJ, Perez SE, Qiao Z, Verdi JM, Hicks C, Weinmaster G, Anderson DJ. Transient Notch activation initiates an irreversible switch from neurogenesis to gliogenesis by neural crest stem cells. Cell 200;101,499 -510. [PubMed]

20. Koch U, Radtke F. Notch and cancer: A double-edged sword. Cell Mol Life Sci 2007;64(21):2746–2762. [PubMed]

21. Jadhav AP, Mason HA, Cepko CL. Notch 1 inhibits photoreceptor production in the developing mammalian retina. Development 2006;133(5):913–923. [Abstract/FREE Full Text]

22. McCright B, Lozier J, Gridley T. Generation of new Notch2 mutant alleles. Genesis 2006;44(1):29–33. [Google scholar]

23. Larrivée B, et al. ALK1 signaling inhibits angiogenesis by cooperating with the Notch pathway Dev Cell 2012;22(3):489–500. [PubMed]

24. Ables JL, Breunig JJ, Eisch AJ, Rakic P.  Not(ch) just development: Notch signalling in the adult brain. Nat. Rev. Neurosci 2011; 12;269-283. [Abstract/FREE Full Text]

25. Artavanis-Tsakonas S, Delidakis C, Fehon RG. The Notch locus and the cell biology of neuroblast segregation. Annu. Rev. Cell Biol 1991; 7:427-452. [PubMed]

26. Fischer A, Schumacher N, Maier M, Sendtner M, Gessler M. The Notch target genes Hey1 and Hey2 are required for embryonic vascular development. Genes Dev 2004;18:901-911. [Abstract/FREE Full Text]

27. Erard CM, Santos CB, Tracey SG. Notch signaling in pathogenesis of diseases. American Journal of BioMedicine 2014;1:19–22. [Abstract/FREE Full Text]

28. Gaiano N, Nye JS, Fishell G. Radial glial identity is promoted by Notch1 signaling in the murine forebrain. Neuron 2000;26: 395-404. [PubMed]

29. Kim TH, Shivdasani RA. Genetic evidence that intestinal Notch functions vary regionally and operate through a common mechanism of Math1 repression. J. Biol. Chem2011; 286: 11427-11433. [Abstract/FREE Full Text]

30. Kopan R, Ilagan MX. The canonical Notch signaling pathway: unfolding the activation mechanism. Cell 2009;137: 216-233. [PubMed]

31. Kopan R, Nye JS, Weintraub H. The intracellular domain of mouse Notch: a constitutively activated repressor of myogenesis directed at the basic helix-loop-helix region of MyoD. Development 1994;120:2385-2396. [Abstract/FREE Full Text]

32. Yousif NG.  Fibronectin promotes migration and invasion of ovarian cancer cells through up‐regulation of FAK–PI3K/Akt pathway. Cell biology international 2014;38(1):85-91. [PubMed]

33. Limbourg F P, Takeshita K, Radtke F, Bronson RT, Chin MT, Liao JK. Essential role of endothelial Notch1 in angiogenesis. Circulation 2005;111:1826-1832. [Abstract/FREE Full Text]

34. Lütolf S, Radtke F, Aguet M, Suter U, Taylor V. Notch1 is required for neuronal and glial differentiation in the cerebellum. Development 2002;129:373-385. [PubMed]

35. Mourikis P, Sambasivan R, Castel D, Rocheteau P, Bizzarro V, Tajbakhsh S. A critical requirement for notch signaling in maintenance of the quiescent skeletal muscle stem cell state. Stem Cells 2012; 30:243-252. [PubMed]

American Journal of Biomedicine © 2017 Frontier Theme
%d bloggers like this: