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Potential effects of gender disparity in downregulation of AKT after post-cerebral ischemia and reperfusion

 
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Research Article  
dx.doi.org/10.18081/2333-5106/015-10/619-630
American Journal of BioMedicine Volume 3, Issue 10, pages 619-630
Published: October 19, 2015


John M. Morrisona, Claire B. Gibson, Zhan Wang, Daniel H. Klionsky mail of corresponding author

Abstract

Cerebral stroke continues to be a major cause of death and the leading cause of long-term disability in developed countries, many studies report gender differences in both mortality and morbidity after cerebral ischemic stroke. No data exists regarding the effect of gender on the cerebral Akt pathway following I/R. We investigated whether sex differences existed in the prevention of stroke through up-uregulation of Akt signaling pathway. In this study, a focal cerebral ischemia/reperfusion injury model was established using middle cerebral artery occlusion in male and female C57BL/6J (wild-type, WT) and Akt knockout (KO) mice. After 24 hours of reperfusion, mice were tested for neurological deficit, cerebral infarct size was assessed and brain tissue was collected. Female WT mice exhibited reduce neurological deficit scores, cerebral infarct size and brain water content in mice with elevated Bcl-2/Bax in the cytoplasm. The gender disparity in the role of Akt in post-ischemic cerebral inflammatory response suggests that Akt signaling activation may have therapeutic potentials in males. 

Keywords: Cerebral stroke; Akt pathway; Bcl-2/Bax; Ischemia/Reperfusion

Copyright © 2015 by The American Society for BioMedicine and BM-Publisher, Inc.

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