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The functionality of cell mediated immunity in the pathogenesis of Schistosoma japonicum

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American Journal of BioMedicine  Volume 2, Issue 7, pages 855-869, July 2014


Wen-jian Chen; Xuefeng Xie; Hao Sakaguchi; Minjun Gao; Men-Bao Joyce; Hong-mei Lei; Jingwei Huang

Abstract

Schistosomiasis japonica is a serious disease caused by the parasitic worm Schistosoma japonicum. The induction of granulomas that form around the eggs in the liver around which are reported to be positively depends upon cell mediated immunity, and there is evidence that increased production of proinflammatory cytokine is associated with strong tissue destruction observed in Schistosomiasis japonica. We investigate the role of regulatory cytokines and cytokine antagonists in the downregulation of immune response in Schistosoma japonicum infection. Male wild type C57BL/6 mice were used, each mouse was infected with 10 cercariae of S. japonicum through the abdominal skin. Single cell suspensions of splenocytes and lymphocytes were prepared by mincing the mouse spleens and mesenteric lymph nodes. IL-10 and TGF-β downmodulate TNF-α and IL-17 production, Neutralization of TNF-α decreased IFN-γ level and the neutralization of IFN-γ decreased TNF-α level and increased IL-10 production. Our study is report that IL-10 and TGF-β are cytokines that appear to be more involved in modulation of immune response in Schistosomiasis japonica.

Keywords: Schistosomiasis japonica; TGF-β; IFN-γ; TNF-α; Cell mediated immunity


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