IL-37 expression improves renal function after ischemia and reperfusion in mice model

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American Journal of BioMedicine Volume 9, Issue 4, pages 207-224

Paloma E. Pinto1,  Aloisio M. Requião-Moura, Felipe D. Ritchie



Renal ischemia is a major problem in the world that lead to renal failure for which no effective treatment is available. Renal ischemia involves a robust inflammatory response, involving up-regulated chemokine expression and leukocyte accumulation, contributes to the mechanism of renal injury and renal failure. IL-37 is a new human cytokine and has an anti-inflammatory function. Currently, it is unknown whether IL-37 suppresses renal inflammatory response to ischemia. We tested the hypothesis that expression of human IL-37 in mouse protects the renal against ischemic injury through suppression of the renal inflammatory response.
IL-37 Tg and WT mice were subjected to right renal nephrectomy to induce unilateral model of ischemia the microvascular clamp was positioned around the left renal pedicles. Serum sampling for measurements of TNF-α, IL-1β, Caspase3, MDA, HMGB1, urea and creatinine. Hematoxylin-eosin staining for histological analysis. The resulted data showed that IL-37 has anti-inflammatory effects in renal IRI as evidenced by significant reduction of the inflammatory markers levels TNF-α, IL-1β and HMGB1. IL-37 has potent antioxidant and anti-apoptotic effects with significant reduction in MDA and caspace-3 respectively.
Keywords: Renal ischemia, IL-37, TNF-α, IL-1β, Caspase-3

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  • Research Article
    American Journal of BioMedicine Volume 9, Issue 4, pages 207-224
    Received September 11, 2021; revised November 24, 2021; accepted December 13, 2021; published December 24, 2021

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    How to cite this article
    Pinto PA, Requião-Moura AM, Ritchie FD. IL-37 expression improves renal function after ischemia and reperfusion in mice model. American Journal of BioMedicine 2021;9(4):207-224.
    Research Article
    1. Abstract
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    3. Introduction
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