Abstract
Interleukin-32 (IL-32) is discovered as proinflammatory cytokine by inducing IL-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α. However, there are unclear data regarding IL-32β associated with worsening of myocardial injury after cardiac ischemia and reperfusion (I/R). In this study, we investigate the prognostic value of IL-32β in inflammatory response after myocardial injury. Anesthetized mice subjected to the myocardial ischemia for 30 min and 2 hours reperfusion. Expression and Regulation of IL-32β were measured by RNA Isolation and Real-Time Polymerase Chain Reaction, inflammatory response in blood and myocardial tissue, were assayed accordingly by ELISA and Western blotting, while Echo for cardiac elements measurement. The I/R group had a significantly higher expression level of IL-32β (0.643±0.012, vs. sham group 0.121±0.013; P<0.05) and associated with worsen myocardial injury, and low cardiac function. In-conclusion, IL-32β might be a new marker associated with adverse event after myocardial injury and may contribute with cardiac remodeling.
Keywords: IL-32β; Polymerase Chain Reaction; Ischemia and Reperfusion
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- Fuster V, Kelly BB, Eds. Promoting cardiovascular health in the developing world. A Critical Challenge to Achieve Global Health Institute of Medicine (US) Committee on Preventing the Global Epidemic of Cardiovascular Disease: Meeting the Challenges in Developing Countries 2010.
- Kim SH, Han SY, Azam T, Yoon DY, Dinarello CA. Interleukin-32: a cytokine and inducer of TNFalpha. Immunity 2005;22(1):131-42.
https://doi.org/10.1016/S1074-7613(04)00380-2
https://doi.org/10.1016/j.immuni.2004.12.003
PMid:15664165 - Netea MG, Azam T, Lewis EC, et al. Mycobacterium tuberculosis induces interleukin-32 production through a caspase- 1/IL-18/interferon-gamma-dependent mechanism. PLoS Med 2006;3(8):e277.
- Shu S, Li CM, You YL, Qian XL, Zhou S, Ling CQ. Electroacupuncture ameliorates cerebral ischemia-reperfusion injury by regulation of autophagy and apoptosis. Evid Based Complement Alternat Med 2016;2016:7297425.
https://doi.org/10.1155/2016/7297425
PMid:27800003 PMCid:PMC5075311 - Kumar A, Haery C, Parrillo JE. Myocardial dysfunction in septic shock. Crit Care Clin. 2000, 16: 251-287.
https://doi.org/10.1016/S0749-0704(05)70110-X - Mjösberg JM. Human IL-25- and IL-33-responsive type 2 innate lymphoid cells are defined by expression of CRTH2 and CD161. Nat Immunol 2011;12(11):1055-1062.
https://doi.org/10.1038/ni.2104
PMid:21909091 - Ohne Y. IL-1 is a critical regulator of group 2 innate lymphoid cell function and plasticity. Nat Immunol 2016;17(6):646-655.
https://doi.org/10.1038/ni.3447
PMid:27111142 - AL Habooby N, Yousif NG, Hadi N, Al-Baghdadi JJ, Vitamin D Attenuates Myocardial Injury by Reduces ERK Phosphorylation Induced by I/R in Mice Model, Current Chemical Genomics and Translational Medicine 2018;12:27-38).
https://doi.org/10.2174/2213988501812010027 - Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF. Protection from RNA and DNA viruses by IL-32. The Journal of Immunology 2011;186(7):4110-4118.
https://doi.org/10.4049/jimmunol.1000081
PMid:21346229 - Kim SH, Han SY, Azam T, Yoon DY, Dinarello CA. Interleukin-32: a cytokine and inducer of TNFalpha. Immunity. 2005;22(1):131-142.
https://doi.org/10.1016/S1074-7613(04)00380-2
https://doi.org/10.1016/j.immuni.2004.12.003
PMid:15664165 - Yousif NG, Al-Amran FG, Hadi N, Lee J, Adrienne J. Expression of IL-32 modulates NF-κB and p38 MAP kinase pathways in human esophageal cancer. Cytokine 2013; 61(1): 223-7.
https://doi.org/10.1016/j.cyto.2012.09.022
PMid:23107826 - Durum R, Memon JF, Mackall TN, et al. Critical role of farnesyltransferase inhibitor in protective myocardial function after endotoxemia in rat model. AmeriJ BioMedi 2015; 2(7): 827-39.
- Grupper R, Edward R, Walter C, et al. Critical role of microRNAs after global myocardial ischemia and reperfusuion. AmeriJBioMedi 2015; 3(7): 451-67.
https://doi.org/10.18081/2333-5106/015-07/451-467 - Yousif NG. Fibronectin promotes migration and invasion of ovarian cancer cells through upregulation of FAK–PI3K/Akt pathway. Cell biol intern 2013; 38(1): 85-91.
https://doi.org/10.1002/cbin.10184
PMid:24115647 - Gysembergh A, Simkhovich BZ, Kloner RA, Przyklenk K. p38 MAPK activity is not increased early during sustained coronary artery occlusion in preconditioned versus control rabbit heart. J Mol Cell Cardiol 2001; 33(4): 681-90.
https://doi.org/10.1006/jmcc.2000.1331
PMid:11273721 - Austin E, Yousif NG, Ao L, et al. Ghrelin reduces myocardial injury following global ischemia and reperfusion via suppression of myocardial inflammatory response. Ameri J BioMed 2013; 1(2): 38-48.
https://doi.org/10.18081/ajbm/2333-5106-013-12/38-48
Yousif NG, Hadi N, Al-Amran F, Zigam Q. Cardioprotective effects of irbesartan in polymicrobial sepsis Herz 2017.
PMid:28144715 - Björklund ÅK, et al. The heterogeneity of human CD127(+) innate lymphoid cells revealed by single-cell RNA sequencing. Nat Immunol 2016;17(4):451-460.
https://doi.org/10.1038/ni.3368
PMid:26878113 - Netea MG, Azam T, Ferwerda G. et al. IL-32 synergizes with nucleotide oligomerization domain (NOD) 1 and NOD2 ligands for IL-1β and IL-6 production through a caspase 1-dependent mechanism. Proceedings of the National Academy of Sciences of the United States of America 2005;102(45):16309-16314.
https://doi.org/10.1073/pnas.0508237102
PMid:16260731 PMCid:PMC1283464 - Yousif NG, Hadi NR, Hassan AM. Indocyanine green-001 (ICG-001) attenuates wnt/β-catenin-induces myocardial injury following sepsis. J Pharmacol Pharmacother 2017; 8(1): 14-20.
https://doi.org/10.4103/jpp.JPP_153_16
PMid:28405131 PMCid:PMC5370323 - Mun SH, Kim JW, Nah SS, et al. Tumor necrosis factor alpha-induced interleukin-32 is positively regulated via the Syk/protein kinase Cdelta/JNK pathway in rheumatoid synovial fibroblasts. Arthritis Rheum. 2009;60(3):678–685.
https://doi.org/10.1002/art.24299
PMid:19248119 - Karolchik D, et al. The UCSC Genome Browser database: 2014 update. Nucleic Acids Res. 2014;42(Database issue):D764-D770.
https://doi.org/10.1093/nar/gkt1168
PMid:24270787 PMCid:PMC3964947 - Kapoor M, Martel-Pelletier J, Lajeunesse D, Pelletier JP, Fahmi H. Role of proinflammatory cytokines in the pathophysiology of osteoarthritis. Nat Rev Rheumatol 2011;7(1):33-42.
https://doi.org/10.1038/nrrheum.2010.196
PMid:21119608 - Yousif NG, Al-Amran FG. Novel Toll-like receptor-4 deficiency attenuates trastuzumab (Herceptin) induced cardiac injury in mice. BMC Cardiovasc Disord 2011;11:62.
https://doi.org/10.1186/1471-2261-11-62
PMid:21999911 PMCid:PMC3209438 - Nah DY, Rhee MY. The inflammatory response and cardiac repair after myocardial infarction. Korean Circ J 2009;39(10): 393-8.
https://doi.org/10.4070/kcj.2009.39.10.393
PMid:19949583 PMCid:PMC2771790 - Vilahur G, Badimon L. Ischemia/reperfusion activates myocardial innate immune response: the key role of the toll-like receptor. Front Physiol 2014;5:496.
https://doi.org/10.3389/fphys.2014.00496
PMid:25566092 PMCid:PMC4270170 - Ferrara JL, Levine JE, Reddy P, Holler E. Graft-versus-host disease (Review). Lancet. 2009;373(9674):1550-1561.
https://doi.org/10.1016/S0140-6736(09)60237-3 - Hong J, Bae S, Kang Y, et al. Suppressing IL-32 in monocytes impairs the induction of the proinflammatory cytokines TNFalpha and IL-1beta. Cytokine 2010;49(2):171-176.
https://doi.org/10.1016/j.cyto.2009.10.003
PMid:19880327 - Netea MG, Azam T, Lewis EC, et al. Mycobacterium tuberculosis induces interleukin-32 production through a caspase- 1/IL-18/interferon-gamma-dependent mechanism. PLoS Med/Public Library of Science 2006;3(8):e277.
- Netea MG, Azam T, Ferwerda G, et al. IL-32 synergizes with nucleotide oligomerization domain (NOD) 1 and NOD2 ligands for IL-1beta and IL-6 production through a caspase 1-dependent mechanism. Proc Natl Acad Sci U S A 2005;102(45):16309-16314.
https://doi.org/10.1073/pnas.0508237102
PMid:16260731 PMCid:PMC1283464 - Nishida A, Andoh A, Shioya M, Kim-Mitsuyama S, Takayanagi A, Fujiyama Y. Phosphatidylinositol 3-kinase/Akt signaling mediates interleukin-32alpha induction in human pancreatic peracinar myofibroblasts. Am J Physiol Gastrointest Liver Physiol 2008;294(3):G831-838.
https://doi.org/10.1152/ajpgi.00535.2007
PMid:18239058 - Zhang C. The role of inflammatory cytokines in endothelial dysfunction. Basic Res Cardiol 2008;103(5):398-406.
https://doi.org/10.1007/s00395-008-0733-0
PMid:18600364 PMCid:PMC2705866 - Yousif NG, Mohammad BI, Hadi N, Al-Dahhan MH, Al-khalidy S. N-acetylcysteine attenuated sepsis-induced cardiac depression: down-regulation of MMP-2 pathway in mice. Pathophysiology of Cell Injury Journal 2016;5:111-125.
- Rasool ST, Tang H, Wu J, et al. Increased level of IL-32 during human immunodeficiency virus infection suppresses HIV replication. Immunology Letters 2008;117(2):161-167.
https://doi.org/10.1016/j.imlet.2008.01.007
PMid:18329725 - Li W, Sun W, Liu L, et al. IL-32. A host proinflammatory factor against influenza viral replication is upregulated by aberrant epigenetic modifications during influenza A virus infection. The Journal of Immunology 2010;185(9):5056-5065.
https://doi.org/10.4049/jimmunol.0902667
PMid:20889550
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