Role of peroxisome proliferator activator receptor-gamma (PPAR-γ) in lung sepsis


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Usha Q Patel 1, Grazia V Clemencet 1, Colleen B Latruffe 2, Peter A Reddy 1, Qin Chu 3, Charlene J Heyman, Alice E Griffin1*
Author Affiliations
∗Correspondence author e-mail: Alice.G11@yahoo.com: 1Department of Medicine, University of Pittsburgh School of Medicine, PA, USA. 2Department of Biochemistry, Faculty of Medicine, Johann Wolfgang Goethe-University Frankfurt, Germany. 3Jinling Hospital, Medical School of Nanjing University, China

 

Abstract

PPAR-gamma has been implicated in the pathology of numerous diseases including; obesity, diabetes, atherosclerosis, and cancer. PPAR-gamma agonists have been used in the treatment of hyperlipidaemia and hyperglycemia. Two isoforms of PPARG are detected in the human and in the mouse: PPAR-γ1 (found in nearly all tissues except muscle) and PPAR-γ2 (mostly found in adipose tissue and the intestine). In the present study, to directly determine the role of PPARγ in lung sepsis we used PPARγ-knockout and C57/BL6 mice model. Mice are treated with Lipopolysaccharide LPS (0.5 mg/kg, iv) for 6-hours, the plasma and tissue cytokines TNF-α, IL-1β, IL-6, IL-10, MIP-1 and KC are analyzed by ELISA. The infiltrations of the mononuclear cells in the lung tissue and degree of lung tissue injury are examined using immunofluorescent and histopathology staining respectively. In PPARγ-knockout mice, LPS induced more expression of pro-inflammatory cytokines expression, which was associated with a marked monocyte infiltration, tissue injury and elevated lung activity of myeloperoxidase compared with wild type C57/BL6 mice. Present study, suggests that PPARγ has a critical role in attenuate lung sepsis and further study need to elucidate the clinical value.

Key words: PPARγ; LPS; Proinflammatory cytokines; Lung; Immunofluorescent; PPARγ-knockout mice

Copyright © 2014 by The American Society for BioMedicine and BM-Publisher, Inc.

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DOI: http://dx.doi.org/10.18081/2333-5106/014-04/228-246
American Journal of BioMedicine 2014, Volume 2, Issue 4, pages 148-169
Received 21 May 2014; accepted September 11, 2014, Published October 22, 2014

How to cite this article
Patel UQ, Clemencet GV, Latruffe CB, Reddy PA, et al. Role of peroxisome proliferator activator receptor-gamma (PPAR-γ) in lung sepsis. American Journal of BioMedicine 2014;2(4):148-169.

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