Study of etiologic multi-drug resistant bacteria of neonatal sepsis in Al-Hilla city

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Amal Talib Atiyah Al-Sa'ady¹∗, Habeeb S. Naher²

   
Abstract
131-blood samples were collected from neonates who were admitted to the preterm units and Neonatal Intensive Care Unit (NICU) in Babylon Hospital for Pediatric and Gynecology. Samples were cultured on many different media, 90(68.7%) of blood samples with positive culture. Among 96 bacterial isolates isolated from these cultures, 40(41.7%) gram negative bacteria versus 56(58.3%) gram positive bacteria. The most frequently isolated bacteria were 13(32.5%) Klebsiella pneumoniae and 11(27.5%) pseudomonas aeruginosa among gram negative. while among gram positive staphylococci have highest rate of isolation 39(69.6%) which included 16(41%) coagulase positive staphylococci and 23(59%) coagulase negative staphylococci (CoNS). Antibiotic susceptibility test performed by disc diffusion test for 28 antibiotics, most isolates have fully resistance to penicillins and cephalosporins. While fully sensitivity was shown in each imipenem and meropenem for both gram negative and gram positive bacteria.

Keywords: Multi-drug resistant bacteria; Neonatal sepsis; Al-Hilla

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References

  1. Carr R, Brocklehurst, P. Doré CJ, Modi N. Granulocyte-macrophage colony stimulating factor administered as prophylaxis for reduction of sepsis in extremely preterm, small for gestational age neonates (the PROGRAMS trial): a single-blind, multicentre, randomized controlled trial. Lancet;2009;373(9659):226-33. [PubMed]
  2. Shahsanam G, Fakoor Z, Karamyyar M, et al. Coagulase Negative Staphy-lococcus; the most common cause of neonatal septicemia in Urmia, Iran. Iranian journal of Pediatrics 2008;18 (3):237-243. [Abstract/Full-Text]
  3. Weinberg G, Powell K. Laboratory aids for diagnosis of neonatal sepsis. In Remington J. Klein J. (Eds.) infectious disease of the fetus and newborn infant. 5th ed., Saunders, Philadelphia: 2001; 1327-1344.
  4. Angus DC, Wax RS. Epidemiology of sepsis. Crit Care Med 2002:29(Suppl):S109-16. [PubMed]
  5. Sundaram V, Kumar P, Narang A. Bacterial profile of early versus late onset neonatal sepsis in a North Indian tertiary care center: Heading towards a change. J. Pediatr Infect Dis 2009;4 :241-5. [PubMed]
  6. Chiesa C, Panero A, Osborn JF, Simonetti AF, Pacifico L. Diagnosis of neonatal sepsis: a clinical and laboratory challenge. Clin Chem 2004;50(2):279-287. [PubMed]
  7. Lee MC, Rios AM, Aten MF, et al. Management and outcome of children with skin and soft tissue abscesses caused by community-acquired methicillin-resistant Staphylococcus aureus. Pediatr. Infect. Dis. J 2004;23 (2):123-127. [PubMed]
  8. Arias E, MacDorman MF, Strobino DM, Guyer B. Annual summary of vital statistics 2002. Pediatrics 2003;112:1215-1230. [PubMed]
  9. Baron EJ, Peterson LR, Finegold SM. Bailey and Scott’s Diagnostic Microbiology. 9th ed. Mosby, Saint Louis, 1994, USA.
  10. Collee JG, Fraser AG, Marmino BP, Simons A. Mackin and McCartney Practical Medical Microbiology.  14th ed., the Churchill Livingstone, Inc., 1996, USA.
  11. MacFaddin JF. Biochemical tests for identification of medical bacteria. 3rd ed. Williams and Wilkins-Baltimor 2000:321-400. [PubMed]
  12. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Amer. J. Clin. Pathol 1996;45:493-496. [PubMed]
  13. Al-Musawy YAS. Detection of gram negative bacteria and yeasts which cause septicemia in children and newborn. MSc thesis. College of Sciences. Al Mustansirya University.
  14. Naher HS, Hasson SO, Al-Mrzoq JM. Gram-Positive Bacteremia in Febrile Children under two years of Age. Int. Res. J. Medical Sci 2013;1(4):6-10. [Abstract/Full-Text]
  15. Baqir KA. Neonatal bacterial sepsis in Babylon province; bacterial etiology, risk factor and treatment. MSc. Thesis. Medicine collage. 2012; Babylon University.
  16. Buttery JP. Blood cultures in newborns and children: optimizingan everyday test. Arch Dis Child Fetal Neonatal Ed 2002;87(1):F25-F28. [PubMed]
  17. Blanco J, Muriel-Bombin A, Sagredo V, et al. Incidence, organ dysfunction and mortality in severe sepsis: a Spanish multicentre study. Crit Care 2002;12:R158. [PubMed]
  18. Lever A, Mackenzie I. Sepsis: definition, epidemiology, and diagnosis. BMJ 2007;335(7625):879-883. [PubMed]
  19. Phua J, Ngerng WJ, See KC, et al. Characteristics and outcomes of culture-negative versus culture-positive severe sepsis. Critical Care 2013;17(R202):1-12. [PubMed]
  20. Al-Talib HI. A bacteriological study in early and late onset neonatal sepsis. M.SC. thesis. Collage of medicine, Al- Mussel university, 2002.
  21. Vincent J, Rello J, Marshall J, et. al. International study of the prevalence and outcomes of infection in intensive care units. JAMA 2009;302(21):2323-329. [PubMed]
  22. Livermore DM, Woodford N. The ß-lactamase threat in Enterobacteriaceae, Pseudomonas and Acinetobacter. Trends Microbiol 2006;14(6):413-420. [PubMed]
  23. Memmel H, Kpwal-Vern A, Latenser B. Infections in diabetic burn patients. Dia. Care 2004;27:229-233. [PubMed]
  24. Al-Faleh KM. Incidence of late onset neonatal sepsis in very low birth weight infants in a tertiary hospital. SQU Medical Journal 2010;10(2):228-30.
  25. Das P, Singh AK, Pal T, Dasgupta S, Ramamurthy T, Basu S. Colonization of the gut with Gram-negative bacilli, its association with neonatal sepsis and its clinical relevance in a developing country. J. Medical Microbiology 2011;60:1651-1660. [PubMed]
  26. Polin RA, Saiman L. Nosocomial infections in the neonatal intensive care unit. Neo Reviews 203;4(3):e81-e89. [Abstract/Full-Text]
  27. Groopman JY. The new generation of resistant infections is almost impossible to treat. Medical Dispatch, Superbugs 2008.
  28. Mesaros N, Nordmann P, Plésiat P. et al. Pseudomonas aeruginosa: resistance and therapeutic options at the turn of the new millennium. Clin Microbiol. Infect 2007;13(6):560-578. [PubMed]
  29. Blanc DS, Francioli P, Zanetti G. Molecular epidemiology of pseudomonas aeruginosa in the intensive care Units, A review. Open Microbiol. J. 2007;1:8-11. [PubMed]
  30. Pourshafie MR, Mousavi SF,  Parzadeh M. Ribotyping and increasing trend of antibiotic resistance of Pseudomonas aeruginosa isolated in Iran. Braz. J. Microbiol 2007;38(3):355. [Abstract/Full-Text]
  31. Kuboyama RH, Oliveira HB, Moretti‐Branchini ML. Molecular epidemiology of systemic infection caused by enterobacter cloacae in a high‐risk neonatal intensive care unit. Infection Control and Hospital Epidemiology 2003;24(7):490-494. [Abstract/Full-Text]
  32. Hervas JA, Ballesteros F, Alomar A, et al. Increase of Enterobacter in neonatal sepsis: a twenty-two-years tudy. Pediatr Infect Dis. J 2001;20(2):134-140. [PubMed]
  33. Antony B, Prasad R. An outbreak of neonatal septicaemia by Enterobacter cloacae. Asian Pacific Journal of Tropical Disease 2011;1(3):227-229. [PubMed]
  34. Loiwal V, Kumar A, Gupta P, Gomber S, Ramachandran VG. Enterobacter aerogenes outbreak in a neonatal intensive care unit. J. Pediatrics International 2002;41(2):157-161. [PubMed]
  35. Narayan SA, Kool JL,  et al. Investigation and control of an outbreak of enterobacter aerogenes bloodstream infection in a neonatal Intensive care unit in Fiji. Infect Control Hosp Epidemiol 2009;30(8):797-800. [PubMed]
  36. Cohen-Wolkowiez M, Moran C, Benjamin DK, et al. Early and late onset sepsis in late preterm infants. Pediatr Infect Dis J 2009;28(12):1052-1056. [PubMed]
  37. Trotman H, Bell Y. Neonatal sepsis in very low birth  weight infants at the University Hospital of the West Indies. West Indian Med J. 2006;55(3):165-9. [PubMed]
  38. Beekmann SE, Diekema DJ, Chapin KC. Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J. Clin. Microbio 2003;41:3119-3125. [PubMed]
  39. Antunes AL, Secchi C, Reiter KC. Evaluation of oxacillin and cefoxitin disks for detection of resistancein coagulase negative staphylococci. Mem Inst Oswaldo Cruz, Rio de Janeiro 2007;102(6):719-723. [Abstract/Full-Text]
  40. Chu A, Hageman JR, Schreiber MD, Alexander K. Antimicrobial therapy and late onset sepsis. Neo Reviews 2012;13(2):e94-e102. [Abstract/Full-Text]
  41. Nash C, Chu A, Bhatti M, Alexander K, Schreiber M,  Hageman  JR. Coagulase Negative Staphylococci in the Neonatal Intensive Care Unit: Are We Any Smarter?. NeoReviews 2013;14(6):e284-e293. [Abstract/Full-Text]
  42. Salyers AA, Whitt DD. Bacterial Pathogenesis: A Molecular Approach, 2nd ed. Washington, D.C. ASM Press, 2002.
  43. Abdallah M, Zaki S, El-Sayed A, Erfan D. Evaluation of secondary bacterial infection of skin diseases in Egyptian in- and outpatients and their sensitivity to antimicrobials. Egypt. Dermatol. Online J 2007;3(2):1-15. [Abstract/Full-Text]
  44. Dancer SJ. The effect of antibiotics on methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother 2008;61(2):246-253. [Abstract/Full-Text]
  45. Deurenberg RH, Vink C, Driessen C, et al. Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR. FEMS Microbiol. Lett 2004; 240(2):225-228. [Abstract/Full-Text]
  46. Garges HP, Moody MA, Cotton CM, et al. Neonatal Meningitis: What Is the Correlation Among Cerebrospinal Fluid Cultures, Blood Cultures, and Cerebrospinal Fluid Parameters? Pediatric 2006;117(4);1094-1101. [PubMed]
  47. Strabelli TM,  Cais DP, Zeigler R, et al. Clustering of Enterococcus faecalis infections in a cardiology hospital neonatal intensive care unit. Braz J Infect Dis 2006;10(2):113-6. [PubMed]
  48. Samuelsson A, Jonasson J, Mosntein HJ, Berg S, Isaksson B. Clustering streptococcal infections in a general intensive care unit. J. HospInfection 2003;54(3):188-95.
  49. Fernandes AT, Ribeiro Filho N, Mazzano RS, et al. Hospital-acquired infection and it's interfaces in the area of health care. São Paulo 2000;14:345-6.
  50. Gilmore M. The Enterococci: Pathogenesis, Molecular Biology and Antibiotic Resistance. Washington, DC: American Society for Microbiology, 2002.
  51. Tendolkar PM, Baghdayan AS, Shankar N. Pathogenic Enterococci: new developments in the 21st century. Cell Mol Life Sci 2003;60(12):2622-36. [PubMed]
  52. Auger S, Ramarao N, Faille C, et al. Biofilm formation and cell surface properties among pathogenic and nonpathogenic strains of the Bacillus cereus group. Appl. Environ. Microbiol 2009;75(20):6616-8. [PubMed]
  53. Jawetz E, Melnick JL, Adelberg EA, Brooks GF. Jawetz, Melnick & Adelberg's Medical microbiology. New York; London, 2001.
  54. Gupta N, Limbago BM, Patel JB, Kallen AJ. Carbapenem-resistant Enterobacteriaceae: epidemiology and prevention. Clin Infect Dis 2011;J1;53(1):60. [PubMed]
  55. Rahman S, Hameed A, Roghani MT, Ullah Z. Multi-drug resistant  neonatal sepsis in Peshawar,Pakistan. Arch Dis Child Fetal Neonatal Ed 2002;87:F52-F54. [PubMed]
  56. Kapoor L, Randhawa VS, Deb M. Microbiological profile of  neonatal septicemia in a pediatric care hospital in Delhi. J.Commun Dis 2005;37(3):227-32. [PubMed]
  57. Saleh RH. Immunological and molecular study on pseudomonas aeruginosa isolated from clinical samples in Babylon Province. Ph.D. Thesis. College of medicine. University of Babylon, 2012.
  58. Köksal N, Hacimustafaoğlu M, Bağci S, Celebi S. Meropenem in severe infections due to multi resistant gram negative bacteria. Indian J Pediatr 2001;68(1):15-19. [PubMed]
  59. Bromiker R, Arad I, Peleg O, Preminger A, Engelhard D. Neonatal bacteremia:patterns of antibiotic resistance. Infect Control Hosp Epide miol 2001;22(12):767-770. [Abstract/Full-Text]
  60. Podschun R, Ullmann U. Klebsiella spp. as Nosocomial Pathogens: Epidem-iology, Taxonomy, Typing Methods, and Pathogenicity Factors. Clinical Microbiology Reviews 2001. [PubMed] 
  61. Jacoby GA, Munoz-Price LS. The new β-lactamases. N. Engl. J. Med 2005;352(4):380-391. [PubMed]
  62. Brook I. Secondary bacterial infections complicating skin lesions. J. Med. Microbiol 2002;51(10):808-812. [PubMed]
  63. Mitscher LA. Bacterial Topoisomerase Inhibitors: Quinolone and Pyridone Antibacterial agents. Chem. Rev 2004;105(2):559-592. [PubMed]
  64. Hussain FM, Boyle-Vavra S, Bethel CD, Daum RS. Current trends in community-acquired methicillin-resistant Staphylococcus aureus at a tertiary care pediatric facility. J. Pediatr.Infect. Dis 2002;19(12):1163-1166. [PubMed]

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DOI: 10.18081/2333-5106/016-11/513-533

Cited by in Scopus

Research Article
American Journal of BioMedicine Volume 4, Issue 11, pages 513-533
Received May 16, 2016; accepted October 26, 2016; published November 29, 2016

How to cite this article
Al-Sa'ady AT, Naher HS. Study of etiologic multi-drug resistant bacteria of neonatal sepsis in Al-Hilla city. American Journal of BioMedicine 2016;4(11):513-533.

Article outline
1. Abstract
2. Keywords
3. Introduction
4. Materials and Methods
5. Results
6. Discussions
7. Acknowledgements
8. References