Detection of some genes resistance to quinolones in pseudomonas aeruginosa isolated from different clinical sources in Al-Diwaniya

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Zainab Falh Dakhl¹*mail of corresponding author, Syoof Khowman Alwan

 
 
Abstract
Pseudomonas aeruginosa is a clinically important pathogen that causes op­portunistic infections and nosocomial outbreaks. Fluoroquinolonesare an important component of antipseudomonal chemotherapy.The major mechanism of resistance of this bacterium to fluoroquinolones is the modification of type II topoisomerases (DNA gyrase and topoisomerase IV). In this study, we examined the mutations in quinolones resistance-determining regions (QRDR) of gyrA, gyrB, parC and parEgenes. A total of 350 clinical isolates were routinely collected. The most common origin of isolates was 162 ear swab followed by 98 burn swab, 50 sputum samples and 40 urine samples. The results of culture and biochemical tests showed that 50 isolate was P. aeruginosa, which then confirmed by api-20E followed by the genotyping detection by 16S rRNA geneusing PCR technique. Antibiotic susceptibility test (by using the Kirby-pour technique) was performed to all these isolates , The results of antibiotic susceptibility test showed that 10 isolate (20%) resistance to at least one type of fluoroquinolones which represented by ciprofloxacin the highest (20%) and norfloxacin (14%) the lowest. The isolates that resistance to at least one type of fluoroquinolones were screened for the presence of genes (gyrA, gyrB, parC and parE ) by polymerase chain reaction. The genesgyrA and parE were found in 10(100%) fluoroquinolones resistant isolates, followed bygyrB4 (40%), parC  2(20%). The most repeated combinations, gyrA+ parE and gyrA+gyrB + parE were detected in (50%) and (30%) of isolates, respectively.

Keywords: P. aeruginosa; Fluoroquinolones; Resistance; DNAgyrase; Topoisomerase IV

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DOI: 10.18081/2333-5106/016-10/427-434

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Research Article
American Journal of BioMedicine Volume 4, Issue 10, pages 427-434
Received July 01, 2016; accepted September 21, 2016; published October 22, 2016

How to cite this article
Dakhl ZF, Alwan SK. Detection of some Genes Resistance to quinolones in pseudomonas aeruginosa Isolated from different clinical sources in Al-Diwaniya. American Journal of BioMedicine 2016;4(10):427-434.

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