Detection of some genes resistance to quinolones in pseudomonas aeruginosa isolated from different clinical sources in Al-Diwaniya

AJBM crossMark

 

 

Zainab Falh Dakhl¹*mail of corresponding author, Syoof Khowman Alwan

 
 
Abstract
Pseudomonas aeruginosa is a clinically important pathogen that causes op­portunistic infections and nosocomial outbreaks. Fluoroquinolonesare an important component of antipseudomonal chemotherapy.The major mechanism of resistance of this bacterium to fluoroquinolones is the modification of type II topoisomerases (DNA gyrase and topoisomerase IV). In this study, we examined the mutations in quinolones resistance-determining regions (QRDR) of gyrA, gyrB, parC and parEgenes. A total of 350 clinical isolates were routinely collected. The most common origin of isolates was 162 ear swab followed by 98 burn swab, 50 sputum samples and 40 urine samples. The results of culture and biochemical tests showed that 50 isolate was P. aeruginosa, which then confirmed by api-20E followed by the genotyping detection by 16S rRNA geneusing PCR technique. Antibiotic susceptibility test (by using the Kirby-pour technique) was performed to all these isolates , The results of antibiotic susceptibility test showed that 10 isolate (20%) resistance to at least one type of fluoroquinolones which represented by ciprofloxacin the highest (20%) and norfloxacin (14%) the lowest. The isolates that resistance to at least one type of fluoroquinolones were screened for the presence of genes (gyrA, gyrB, parC and parE ) by polymerase chain reaction. The genesgyrA and parE were found in 10(100%) fluoroquinolones resistant isolates, followed bygyrB4 (40%), parC  2(20%). The most repeated combinations, gyrA+ parE and gyrA+gyrB + parE were detected in (50%) and (30%) of isolates, respectively.

Keywords: P. aeruginosa; Fluoroquinolones; Resistance; DNAgyrase; Topoisomerase IV

Copyright © 2016 by The American Society for BioMedicine and BM-Publisher, Inc.

Article citationReferencesFull-Text/PDFFeedback
The citation data is computed by the following citation measuring services:

Google scholarcitedby

References

  1. Gawish A, Mohammed N, El-Shennawy G, Mohammed H. An investigation of type 3 secretion toxins encoding-genes of Pseudomonas aeruginosa isolates in a university hospital in Egypt. J. of Microbio. And Infec. Dise 2013;3(3):122-116. [Abstract/Full-Text]
  2. Cicek AC, Saral A, Duzgun AO, et al. Screening of class 1 and class 2 integrons in clinical isolates of Pseudomonas aeruginosa collected from seven Hospitals in Turkey: A Multicenter Study. J. Med. Microb 2013;3:233-227. [Abstract/Full-Text]
  3. Anvarinejad M, Farshad S, Alborzi A, Ranjbar R, Giammanco G, Japoni A. Integron and genotype patterns of Quinolones- resistant uropathogenic Escherichia coli .Afr. J.Microbial. Res Italy 2011;5(22):3770-3765. [PubMed]
  4. Cho HH, Kwon KC, Kim S, Koo SH. Correlation between virulence genotype and fluoroquinolone resistance in carbapenem-resistant Pseudomonas aeruginosa. Original ArticleClinical Microbiology 2014;34:292-286. [Abstract/Full-Text]
  5. Ghosh UK, Hore A. Isolation and Characterization of Nalidixic Acid Resistant Mutant of a Thermophilic Actinomycete. Int. J. Microbiol. Res 2011;2:123-120. [Abstract/Full-Text]
  6. Salma RM, Dabboussi FA, Kassaa IO, Khudary RH, Hamze MM. gyrA and parC Mutations in Quinolone-Resistant Clinical Isolates of Pseudomonas aeruginosa from Nini Hospital in North Lebanon. International Journal of Microbiological Research 2012;3(2):86-82. [Abstract/Full-Text]
  7. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing; 22nd. Informational Supplement 2012;32(3). [Abstract/Full-Text]
  8. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989, N.Y.
  9. Bartlett JMS, Stirling D. PCR Protocols: Methods in molecular biology. 2nd. Humana Press Inc 1998, Totowa.
  10. Jamshaid A K, Zafar I, Saeed U R, Kalsoom F, Abbas K. Prevalence and resistance pattern of Pseudomonas aeruginosa against various antibiotics. Pak J Pharm Sci 2008;21(3):315-311. [Abstract/Full-Text]
  11. Aziz Z S. Study of carbapenems phenotypic resistance in Pseudomonas aeruginosa isolated from burns in Misan province. Journal of International Academic Research for Multidisciplinary 2015;3(3):5083- 2320.
  12. Upadhaya S, Shenoy R, Shetty V, Lamsal A, Lamichhane P, Pokhrel S. Multi-drug Resistant Pseudomonas aeruginosa Isolated from Intensive Care Burn Unit. International Journal of Biomedical Research 2014;5(4):9763-9633. [Abstract/Full-Text]
  13. Avains AB. Identification of unusal pathogenic gram-negative aerobic and an aerobic. Afr. J. Med. Sci 2009;24:139-135.
  14. Al-juboriSS, Al-Jabiri HA, Al-Kadmy IMS. Molecular Detection of Aminoglycoside Resistance Mediated by Efflux Pump and Modifying Enzymes in Pseudomonas aeruginosa Isolated From Iraqi Hospitals. Int'l Conf. on Medical Genetics, Cellular & Molecular Biology, Pharmaceutical & Food Sciences 2015 June 5-6, Istanbul.
  15. Al-Shara JMR. Phenotypic and molecular detecting of carbapenem resistant Pseudomonas aeruginosa in Najaf Hospitals. Ph.D. Thesis. Faculty of Science 2013. University of Kufa. Iraq.
  16. Al-Muhannak FH. Spread of some extended-spectrum beta-lactamases in clinical isolates of Gram-negative bacilli in Najaf. M.Sc. Thesis.College of Medicine 2010.University of Kufa.
  17. Al-Kabei MNH. Isolation and Characterization of Lytic Bacteriophages Infecting Pseudomonas aeruginosa. M.Sc. Thesis. College of Sciences 2009. Al-Qadisiya University.
  18. DeMiguel Martinez I, Del Rosario Quintana C, Bolanos Rivero M, Ramos Macias A. Aetiology and therapeutic considerations in chronic otitis media. Analysis of a 5 years period. Acta. Otorrinolaringol. Esp 2005;56(10):462-459.
  19. Belal EJK. Investigation of some β-lactamases in clinical isolates of Pseudomonas aeruginosa in Najaf City. M.Sc. Thesis. Education of Girls 2010. University of Kufa. Iraq.
  20. Gasink LB, Fishman NO, Weiner MG, Nachamkin I, Bilker WB, Lautenbach E. Fluoroquinolone-resistant Pseudomonas aeruginosa: assessment of risk factors and clinical impact. Am. J. Med 2006;119:25-19. [PubMed]
  21. Jalal S, Ciofu O, Hoiby N, Gotoh N, Wretlind, B. Molecular mechanisms of fluoroquinolone resistance in Pseudomonas aeruginosa isolates from cystic fibrosos. Antimicrob. Agents Chemother 2000;44(3):712-710. [PubMed]
  22. Ghosh UK, Hore A. Isolation and Characterization of Nalidixic Acid Resistant Mutant of a Thermophilic Actinomycete. Int. J. Microbiol. Res 2011;2:123-120. [Abstract/Full-Text]
  23. Aeschlimann JR. The role of multidrug efflux pumps in the antibiotic resistance of Pseudomonas aeruginosa and other Gram-negative bacteria. Insights from the Society of Infectious Diseases Pharmacists. Pharmacotherapy 2003;23:924-916.
  24. Agnello M, Wong-Beringer A. Differentiation in quinolone resistance by virulence genotype in Pseudomonas aeruginosa. PLoSOne 2012;7(8):e42973. [PubMed]
  25. Rieuwpassa IE, Dwiyanti R, Yasir Y, Sabir M, Hatt M. Topoisomerases II mutations in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa in Makassar, Indonesia. American Journal of Infectious Diseases and Microbiology 2015;3 (3):94-91. [Abstract/Full-Text]
  26. Yang X, Xing B, Liang C, Ye Z, Zhang Y. Prevalence and fluoroquinolone resistance of pseudomonas aeruginosa in a hospital of South China. Int J Clin Exp Med 2015;8(1):1390-1386. [PubMed]
  27. Matsumoto M, Shigemura K, Shirakawa T, Nakano Y, Miyake H, Tanaka K. Mutations in the gyrA and parC genes and in vitro activities of fluoroquinolones in 114 clinical isolates of Pseudomonas aeruginosa derived from urinary tract infections and their rapid detection by denaturing high-performance liquid chromatography. Int J Antimicrob Agents 2012;40:444-440. [PubMed]

READ THE FULL ARTICLE

1. Access this article through OpenAthens

2. Access this article through your login credentials/Subscription

Get Access

3. Purchase this article at rate $55.00 and received Full-Text/PDF
You will have online immediate access to article following the completion of this purchase and you may download and print a copy of each article for your personal use. Use the coding below to purchase your article as PDF by credit card, debit card, will be asked to supply your billing card information. Before continue with your purchase please read carefully the BM-Publisher terms and conditions of purchase.

Purchase Article

For any technique error please contact us and will be response to sending purchase article by email.

Thank you for visiting American Journal of BioMedicine. * = Required fields

Error: Contact form not found.

Print Friendly, PDF & Email
Downloads: 2,611

DOI: 10.18081/2333-5106/016-10/427-434

Cited by in Scopus

Research Article
American Journal of BioMedicine Volume 4, Issue 10, pages 427-434
Received July 01, 2016; accepted September 21, 2016; published October 22, 2016

How to cite this article
Dakhl ZF, Alwan SK. Detection of some Genes Resistance to quinolones in pseudomonas aeruginosa Isolated from different clinical sources in Al-Diwaniya. American Journal of BioMedicine 2016;4(10):427-434.

Service

Email this link to a friend
Add article to my folders
Subscribe/Renew
Alert me when this article is cited
Add to portfolio
Contact with editor
Advertising