Critical role of IL-32 invasion and metastasis of colonic cancer through upregulation of matrix metalloproteinase (MMP)-2

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Abstract

Interleukin (IL)-32 is proinflammatory cytokine, which has been shown to play an important role in cancer growth and metastasis. Here, we investigated whether the proinflammatory cytokine IL-32 may be involved in colonic carcinogenesis through upregulation of matrix metalloproteinase (MMP)-2. We are obtaining consent from all patients, 136 patients (88 males and 48 females; median age, 57 years; range 38–8 years) diagnosed patholgically with colonic cancer from 2014 to 2017 were participated in the study. Tumor tissue and adjacent noncancerous mucosa were harvested immediately following colonic resection. Quantitative reverse transcription PCR (qRT-PCR) was performed using SYBR green. Formalin-fixed and paraffin-embedded tissues were examined with immunohistochemistry (IHC) using primary antibody. Our results clearly suggest that IL-32 is an important mediator for colonic cancer metastasis and independent prognostic predictor of colonic cancer. Moreover, IL-32-facilitated cell migration and invasion in vitro was mediated through upregulation of matrix metalloproteinase (MMP)-2 expression. These data demonstrate that IL-32 plays a critical role in invasion and metastasis of colon cancer.

Keywords: IL-32; Colonic cancer; PCR; Matrix metalloproteinase (MMP)-2

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Research Article
DOI: http://dx.doi.org/10.18081/2333-5106/018-10/661-684
American Journal of BioMedicine Volume 6, Issue 8, pages 661-684
Received July 28, 2018; accepted October 16, 2018; published October 24, 2018

How to cite this article
Tsai C, Kim H, Adriennea J. Critical role of IL-32 invasion and metastasis of colonic cancer through upregulation of matrix metalloproteinase (MMP)-2. American Journal of BioMedicine 2018;6(10):661-684.

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