Polymorphism of BRAF gene in colorectal cancer in Iraqi patients

Research Article

dx.doi.org/10.18081/2333-5106/016-5/81-89

American Journal of BioMedicine Volume 4, Issue 5, pages 81-89

Received February 05, 2016; accepted April 12, 2016; published May 26, 2016

Enas Muhsin Abbass^a, Estabraq AR Al-Wasiti^b, Alaa Ghani Hussein^c

a Department of Clinical BioChemistry, College of Medicine, Al- Mustansiriyah University. b Department of Clinical Bio Chemistry, College of Medicine, Al- Nahrain University. c Department of pathology, College of Medicine, Al- Nahrain University

Abstract

Colorectal cancer (CRC) is one of the leading causes of cancer morbidity and mortality. BRAFV600E is the single nucleotide polymorphism and most important mutation of this gene lead to development cancer. It shows variable frequency in Colorectal cancer. This molecular bases study was focused on the BRAFV600E and the nucleotide variation in patients. Seventy patient samples and 10th healthy participates as control. Patient were 25-80 year-old with mean age 52.5 year-old. DNA was extracted and amplified by conventional PCR technique and 65Amplicons were sequenced using Sanger method. There is no detection of BRAFV600E mutation in all investigated cases, but the molecular investigation of BRAF fragment obtain several nucleotide polymorphisms two of them T>C 145 and 161 were common in both healthy and patient groups. Other nucleotide variation was detected in the patients group only contained 179 T/C frequency was100% all patient’s groups followed by 218 T/C (83.07%) and 207 T/C (46.15%) in other patient’s groups.

Keywords: BRAFV600E; Transition; Colorectal Cancer; SNP