Adhesion molecule expression trigger immune-mediated pathology in lupus-nephritis

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Bassim Jens Boldron 1, Simon Caltabiano 1, Steven D. Debono 1, 2, Robert Thompson 1, Malcolm Scammells 1, Robert Westover 1, Ye Wu 1, 3, Philip Frugier *2 
Author Affiliations
∗Correspondence author e-mail:Philip.Frugier @qmail.com: 1Department of Internal Medicine, The Ohio State Wexner Medical Center, Ohio, USA, 2Centre for Inflammatory Diseases, Monash University Department of Medicine, Australia , 3Core Research Laboratory, School of Medicine, Xi'an Jiaotong University, Xi'an, China.

 

Abstract

Doxorubicin (Dox) is one of the most potent broad-spectrum antitumor anthracycline antibiotics, its use is limited by the development of life-threatening cardiomyopathy. Doxorubicin generates free radicals and induces oxidative stress associated with cellular injury. Further, it has been shown that free radicals are involved in doxorubicin-induced toxicity. The goal of this study is to investigated the cardio-protective effects of caffeic acid on doxorubicin induced cardiotoxicity. The rats were randomized into three equal groups, sham group without treatment, doxorubicin treated group at a dose 3mg/kg IP every other two days and group treated with doxorubicin plus caffeic acid 40mg/day. Two weeks later LV function measurment were performed and blood samples were collected from the heart to measurment plasma levels of cardiac Troponin-I (cTn-I), oxidative stress parameter malondialdehyde (MDA) and high a sensitive c-reactive protein (hs-CRP). The hearts were excised for cardiac tissue cytokines (TNF-α, IL-1β, IL-10) measurement and microscopic examination. Rats in the Dox+caffeic acid group had improved LV function, reduced cytokine expression, decreased myocardial marker injury (cTn-I) and less MDA, hs-CRP levels in comparison with the Dox group. Pathological finding appeared nearly normal in the Dox+caffeic acid without fibrosis. The results of the present study reveal that caffeic acid has a promising cardioprotective effect against doxorubicin-induced cardiotoxicity.

Keywords: Doxorubicin; Cardiotoxicity; Inflammatory response; Left ventricular function

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Research Article
DOI: http://dx.doi.org/110.18081/ajbm/2333-5106-013-12/49-57
American Journal of BioMedicine Volume 2, Issue 1, pages 27-36
Received 02 September 2013; accepted 17 January 2014; published 22 February 2014

How to cite this article
Boldron J, Caltabiano S, Debono SD, Thompson R, Scammells M,Westover R, Wu Y, Frugier P. Adhesion molecule expression trigger immune-mediated pathology in lupus-nephritis. American Journal of BioMedicine 2014;2(1):27-36.

Case report outline
1. Abstract
2. Keywords
3. Introduction
4. Methods
5. Results
6. Discussion
7. References