“Research Article”
Received August 09, 2016; Accepted December 30, 2016; Published January 21, 2017
http://dx.doi.org/10.18081/2333-5106/017-16-31
Asraa NA. Alzubaidi; Ziad MF. Alkozai
Abstract
This study aimed to prepare an experimental nano vaccine for Acinetobacter baumannii. In current study, Acinetobacter isolates were identified by growth onto CHROMagarTM Acinetobacter/MDR and standard biochemical tests. Identification of isolates of Acinetobacter isolates to species level was done by VITEK 2 compact system and API20. Chitosan nanoparticles was used in this study as an adjuvant, the results showed chitosan a perfect adjuvant because it carried outer membrane protein antigen and stimulates an immune response at treated rats, use of Chitosan nanoparticles as a carrier to OMP increased the concentration of cytokines (IL-2,IL-6,IFN-ƴ), antibody titer, total leukocytes and differential leukocytes in treated rats compared with each control group and OMP group.
Keywords: Vaccine; IL-2; IL-6; INFƴ; WBCs count
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