Methionine protects from myocardial ischemia/reperfusion injury via down regulation of the inflammatory response and apoptosis

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Najah R. Hadi^*1, Fadhil G. Al-Amran ², Mohammad D. Alrekabi ¹, Maitham G Yousif ², Furqan H. Hussein ¹

∗Correspondence author e-mail: Daniel _D @yahoo.com: ¹Department of pharmacology & therapeutics, Faculty of medicine, University of Kufa, ²Department of cardiothoracic surgery, Faculty of medicine, University of Kufa, ³Department of science, Fculty of Science, Al-Qadisiyah

Abstract

Ischemia-reperfusion of cardiac tissues may lead to a prominent damage of the myocyte through either necrosis or apoptosis that seems to be the predominant modes of death during this period. In this study, we investigated the effects of L-Methionine in regional ischemia/ reperfusion injury and apoptosis. Dwale-sprague rats were divided into four groups (six rats per group). Sham group, rats were subjected for all surgical procedure without ligation of the left interior descending coronary artery (LAD). Control group, in which LAD was ligated. Control vehicle and L-methionine treated groups, rats pretreated with normal saline and L-methionine (100 mg/kg, IP ), respectively, for 7 days then subjected to the surgical procedure with ligation of LAD for 25 minutes followed by 120 minutes reperfusion. At the end of reperfusion, cardiac tissue TNF-α, IL-1β, IL-6 and ssDNA, as well as plasma cardiac troponin I (cTnI) were measured. It has been found that L-methionine treated group showed significant reduction (P˂0.05) in TNFα, IL-1β, IL-6, ssDNA and cTnI with respect to the control groups. Histopathology study revealed that the treatment with L-methionine significantly (P˂0.05) improved cardiac injury as compared with control groups and the total severity scores showed that the cardiac injury was mild (score 1) in 50.0%, moderate (score 2) in 33.3% and sever (score 3) in 16.7% of L-methionine treated group. It is concluded that L-methionine reduces inflammatory reaction associated with ischemia/reperfusion injury induced by LAD ligation in addition to its reduction for cardiac injury induced by ischemia reperfusion.

Key words: Methionine; Ischemia/reperfusion; Ghrelin; cTn-I; Pro-inflammatory cytokines

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