Advertisement

NF-kappaB dependent inflammatory response modulates by Toll-like receptor in sepsis

 

"Research Article"

American Journal of BioMedicine  Volume 3, Issue 3, pages 134-140, March 2015


Delbert N.Thota; Anna Oddis; Gurpreet Banerjee; Hiroshi Caudell, Roschelle Ohura

Abstract

Toll-like receptor 4 (TLR4), as a membrane surface receptor for bacterial, fungal, viral and certain endogenous substances, has been described to contribute to the development of sepsis. Wild-type mice and HJN mice were treated with intraperitoneal lipopolysaccharide (LPS) then serum and tissue from myocardium, lung and kidney analysis proinflammatory response using double immunofluorescence, histopathology stain, western blott, and ELISA. We repeated this experiment in chimeric mice lacking NF-kappaB expression. From these experimental data, the TLR4 is overactivated in sepsis and induce proinflammatory response through upregulation of NF-kappaB expression. Further experimental research is needed, especially of the study of biochemical markers of the injury process to establish the role of TLR4 in sepsis.

Keywords: Sepsis; TLR4; lipopolysaccharide; NF-kappaB


Limited Access             Full Text-PDF                Feedback


References

1. Rangel-Frausto MS, Pittet D, Costigan M, Hwang T, Davis CS, Wenzel RP. The natural history of the systemic inflammatory response syndrome (SIRS). A prospective study. JAMA 1995; 273:117–123. [PubMed]

2. Russell JA, Singer J, Bernard GR, et al. Changing pattern of organ dysfunction in early human sepsis is related to mortality. Crit. Care Med 2000;28: 3405–3411. [PubMed]

3. Akira S, Takeda K, Kaisho T. Toll-like receptors: critical proteins linking innate and acquired immunity.Nat Immunol 2001;2:675–680. [PubMed]

4. Xing L, Remick DG. Relative cytokine and cytokine inhibitor production by mononuclear cells and neutrophils. Shock 2003;20:10–16. [PubMed]

5. Tavener SA, Kubes P. Is there a role for cardiomyocyte toll-like receptor 4 in endotoxemia? Trends Cardiovasc Med 2005;15:153–157. [PubMed]

6. Hoste EA, Lameire NH, Vanholder RC, et al. Acute renal failure in patients with sepsis in a surgical ICU: predictive factors, incidence, comorbidity and outcome. J. Am. Soc. Nephrol 2003; 14:1022–1030. [PubMed]

7. Schrenk AH; Abe R; Scammells M; Frugier P. Protects against cell injury and infection: activation of immune system/AhR. Pathophysiology of Cell Injury Journal 2014; 2:42-54. [Abstract/Full-Text]

8. Prabhu SD. Cytokine-induced modulation of cardiac function. Circ Res 2004;95:1140–1153. [PubMed] [Cross Ref]

9. Weisensee D, Bereiter-Hahn J, Schoeppe W, Low-Friedrich I. Effects of cytokines on the contractility of cultured cardiac myocytes. Int J Immunopharmacol 1993;15:581–587. [PubMed]

10. Kumar A, Haery C, Parrillo JE. Myocardial dysfunction in septic shock. Crit Care Clin 2000;16:251–287. [PubMed]

11. Mittal R, Coopersmith CM. Redefining the gut as the motor of critical illness. Trends Mol Med 2014;20:214–223. [PubMed]

12. Slimani H, Zhai Y, Yousif NG, et al. Enhanced monocyte chemoattractant protein-1 production in aging mice exaggerates cardiac depression duringendotoxemia. Crit Care 2014;18(5):527. [PubMed]

13. Kobayashi Y. The role of chemokines in neutrophil biology. Front Biosci 2008;13:2400–2407. [PubMed]

14. Deshmane SL, Kremlev S, Amini S, Sawaya BE. Monocyte chemoattractant protein-1 (MCP-1): an overview. J Interferon Cytokine Res 2009;29:313–326. [PubMed]

15. Adams DH, Harvath L, Bottaro DP, et al. Hepatocyte growth factor and macrophage inflammatory protein 1 beta: structurally distinct cytokines that induce rapid cytoskeletal changes and subset-preferential migration in T cells. Proc Natl Acad Sci USA 1994;91:7144–7148. [PubMed]

16. Austrup F, Vestweber D, Borges E, et al.  P- and E-selectin mediate recruitment of T-helper–1 but not T-helper–2 cells into inflamed tissues. Nature 1997;385:81–83. [PubMed]

17. Hyduk SJ, Chan JR, Duffy ST,et al. Phospholipase C, calcium, and calmodulin are critical for alpha4beta1 integrin affinity up-regulation and monocyte arrest triggered by chemoattractants. Blood 2007;109:176–184. [PubMed]

18. Levy MM, Dellinger RP, Townsend SR, et al. The Surviving Sepsis Campaign: results of an international guideline-based performance improvement program targeting severe sepsis. Crit Care Med 2010; 38(2):367-74. [PubMed]

19. Angus DC, Linde-Zwirble WT, Lidicker J, Clermont G, Carcillo J, Pinsky MR. Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care. Crit Care Med 2001; 29(7):1303-10. [PubMed]

 

 

American Journal of Biomedicine © 2017 Frontier Theme
%d bloggers like this: