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A study of anti-diabetic activity of gensinoside by assessing sodium-glucose symporter blocking effect in silico and in rat intestinal model

American Journal of BioMedicine  Volume 2, Issue 8, pages 984-991, August 2014


 Sahar A. majeed; Heider S Qassam; Karrar Kareem Gaen; Khalida Kadhim; Fadhaa Abdul Ameer; Ikhlas Hassan; Hussein Abdul Kadhim

Abstract

Diabetes mellitus has repeatedly attracted attention of researchers to design different therapeutic approaches to achieve an optimal treatment for this serious health challenge. The purpose of the study is to evaluate treatment option for controlling hyperglycemia at site of glucose absorption through designing computerized and in vivo models to test a sodium-glucose symporter based drug design. In silico protein data bank (pdb) model of SGLUT was processed and analyzed for docking with edited test glycoside. Another model included determination of the dose-response relationship in rat intestinal glucose/saline perfusion with test glycoside. Our result showed that Ginsenoside revealed a dose dependent SGLUT blocking activity in a saturation kinetics curve, which was agreed with in silico model results.

Keywords: SGLUT; Gensinoside; Perfusate glucose; ICM docking


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