Musaed Hekmat Aldhhan
Abstract
The elusive mechanism of spinal cord ischemia-reperfusion injury has delayed the development of pharmacological adjuncts. The objective of this study is to investigate the neuroprotective role of biglycan in spinal injury following I/R after aortic occlusion via down-regulation of ICAM-1 involving ERK1/2 pathway. Adult male Albino rats were underwent aortic occlusion for 5 minutes, followed by 48 hours of reperfusion when spinal cords were removed for analysis. Rat treated with biglycan attenuated ICAM-1 expression with down-regulation of ERK1/2 pathway. Further; biglycan decreased cytokine production and had preserved functional outcomes and neuronal viability after thoracic aortic occlusion. In conclusion, biglycan attenuated neuronal injury after thoracic aortic occlusion in rat through decreased the activity of ERK1/2 pathway that significantly attenuated the production of proinflammatory cytokines.
Keywords: Biglycan; ICAM-1; ERK1/2; Spinal cord I/R
References
1. Cooley D, Golino A, Frazier O. Single-clamp technique for aneurysms of the descending thoracic aorta: report of 132 consecutive cases. Eur J Cardio-thorac Surg 2000; 18:162–167. [PubMed]
2. Tuttolomondo A, Di Raimondo D, di Sciacca R, Pinto A, Licata G. Inflammatory cytokines in acute ischemic stroke. Curr Pharm Des 2008;14:3574–3589. [PubMed]
3. Mirabelli-Badenier M, Braunersreuther V, Viviani GL, et al. CC and CXC chemokines are pivotal mediators of cerebral injury in ischaemic stroke. Thromb Haemost 2011; 105:409–420. [PubMed]
4. Tuttolomondo A, Di Sciacca R, Di Raimondo D, Renda C, Pinto A, Licata G. Inflammation as a therapeutic target in acute ischemic stroke treatment. Curr Top Med Chem 2009; 9:1240–1260. [PubMed]
5. Griepp R, Ergin M, Galla J, Klein J, Spielvogel D, Griepp E. Minimizing spinal cord injury during repair of descending thoracic and thoraco-abdominal aneurysms. Semin Thorac Cardiovasc Surg 1998;10:25–28. [PubMed]
6. Roughley PJ, White RJ. Dermatan sulphate proteoglycans of human articular cartilage. The properties of dermatan sulphate proteoglycans I and II. The Biochemical Journal 2000: 262(3): 823–7. [PubMed]
7. Roughley PJ, White RJ, Magny MC, Liu J, Pearce RH, Mort JS. Non-proteoglycan forms of biglycan increase with age in human articular cartilage. The Biochemical Journal 1996; 295(2): 421–6. [PubMed]
8. Bock HC, Michaeli P, Bode C, et al. The small proteoglycans decorin and biglycan in human articular cartilage of late-stage osteoarthritis. Osteoarthritis and cartilage 2001; 9: 654 – 663. [PubMed]
9. Rafii MS, Hagiwara H, Mercado ML, et al. Biglycan binds to a- and g-sarcoglycan and regulates their expression during development. Journal of cellular physiology 2006;209:439 – 447. [PubMed]
10. Lang-Lazdunski L, Matsushita K, Hirt L, et al. Spinal cord ischemia: development of a model in the mouse. Stroke 2000;31:208–213. [PubMed]
11. Xiang H, Wael S, Jong-Ho K, et al. IL-37: Novel neuroprotective effects after brain ischemia and reperfusion. American Journal of BioMedicine 2014; 2(1):113-123. [Abstract/Full-Text]
12. Yousif NG, Al-amran F. Novel Toll-like receptor-4 deficiency attenuates trastuzumab (Herceptin) induced cardiac injury in mice. BMC cardiovascular disorders 2011;1: 62. [PubMed]
13. Coselli J, LeMaire S. Left heart bypass reduces paraplegia risk after thoraco-abdominal aortic aneurysm repair. Ann Thorac Surg 1999; 67:1931–1934. [PubMed]
14. Carrel T, Berdat P, Robe J, et al. Outcomes of thorac-abdominal aortic operations using deep hypothermia and distal exsangunination. Ann Thorac Surg 2000; 69:692–695. [PubMed]
15. Kouchoukos N, Dailey B, Rokkas C, Murphy S, Bauer S, Abboud N. Hypothermic bypass and circulatory arrest for operations on the descending thoracic and thoracoabdominal aorta. Ann Thorac Surg 1995; 67–76. [PubMed]
16. Mirabelli-Badenier M, Braunersreuther V, Viviani GL, et al. CC and CXC chemokines are pivotal mediators of cerebral injury in ischaemic stroke. Thromb Haemost 2011;105:409–420. [PubMed]
17. Dinarello CA. A clinical perspective of IL-1β as the gatekeeper of inflammation. Eur J Immunol 2011;41: 1203–1217. [PubMed]
18. Zong S, Zeng G, Wei B, Xiong C, Zhao Y. Beneficial effect of interleukin-1 receptor antagonist protein on spinal cord injury recovery in the rat. Inflammation 2012; 35:520–526. [PubMed]
19. Shikama Y, Aki N, Hata A, Nishimura M, Oyadomari S, Funaki M. Palmitate-stimulated monocytes induce adhesion molecule expression in endothelial cells via IL-1 signaling pathway. J Cell Physiol 2015;230(3):732-42. [PubMed]
20. Taira Y, Marsala M. Effect of proximal arterial perfusion pressure on function, spinal cord blood flow, and histopathologic changes after increasing intervals of aortic occlusion in the rat. Stroke 1996;27(10):1850-8. [PubMed]
21. Colton CA. Microglial-neuronal interactions during neurodegenerative diseases. J Neuroimmune Pharmacol 2013;8:4–6. [PubMed]
22. Fang B, Li XM, Sun XJ, et al. Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption. Int J Mol Sci 2013;14:10343–10354. [PubMed]
23. HJiang XH, HAi CH, HShi EH, HNakajima YH, HMa HH. Neuroprotection against spinal cord ischemia-reperfusion injury induced by different ischemic postconditioning methods: roles of phosphatidylinositol 3-kinase-akt and extracellular signal-regulated kinase. Anesthesiology 2009;111:1197–1205. [PubMed]
