Magnesium sulfate ameliorates cerebral ischemia reperfusion injury via interfering with inflammatory and oxidative pathways

American Journal of BioMedicine  Volume 2, Issue 9, pages 1079-1094 September 2014

Najah R Hadi, Ahmed M Hasan, Zahraa K Al-Hassani, Mohamed Al-Ameri, Nasser G. Yousif


Magnesium sulfate has neuroprotective effects and decrease overall neuronal firing. It is also decrease firing of excitable tissues outside the brain. We study the anti-inflammatory and antioxidant effects of magnesium sulfate in rat brain following  ischemia reperfusion (I/R) stress. Twenty four rats were grouped into 4 groups: The first (sham group), the second (control) and the third group(control-vehicle) and the forth  (treated with Magnesium sulfate). Animals in the second group underwent bilateral common carotid artery ligation without treatment, whereas the forth group were injected with magnesium sulfate 250mg/kg intraperitoneally before procedure. Brain homogenate  were prepared after the procedure for measurement of cerebral  level of IL-6, IL-9, MCP-1 and ICAM. Cerebral level of IL-9 in control group was 163.3 ± 30.4 pg/mg and it significantly decreased in magnesium sulfate treated group (21.8 ± 1.72 pg/mg). Cerebral level of MCP-1 in the control group was 109.05 ± 18.2 pg/mg, while  it significantly reduced in magnesium sulfate treated group (38.16 ± 3.54 pg/mg). Mean cerebral levels of ICAM of control was 362.8 ± 26.81 pg/mg while mean cerebral level of ICAM in treated group was 35.5 ± 4.71 pg/mg. We concluded that magnesium sulfate significantly decreasedcerebral inflammatory markers IL-6, IL-9, MCP-1 and ICAM in global ischemia model in rats and regressed I/R injury

Keywords: Magnesium sulfate; Global cerebral ischemia; IL-9;  ICAM; MCP-1

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