Notch-3 promote angiogenesis and proliferation of bladder cancer cells through the PI3K/Akt pathway

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American Journal of BioMedicine  Volume 2, Issue 6, pages 724-731, June 2014

Joan V. Draucker; Irene Talarico; Ling Wu; David B. Mulier; Xian Chen; Celeste B. Determan; Jean Friese; Ling Yao


Transitional cell carcinoma (TCC) of the bladder is the most common form of bladder cancer and is manifested in two distinct forms with different clinical and biological behaviors. Approximately 70% of patients present with non-muscle invasive tumors, while the remaining 30% present with muscle-invasive tumors. Despite good prognosis for patients with superficial disease, recurrence is common and is associated with development of muscle-invasive disease. Seventy seven tissue specimens from bladder cancer patient [32- noninvasive and 45- muscle invasive] and forty specimens from patients with benign prostatic hypertrophy as control were enrolled. The specimen’s tissue was halved into either in 10% neutral buffered formalin for histological process or snap frozen storage in liquid nitrogen. Tissue RNA and protein were examined by qPCR and western blot for Noch-3 and PI3K/Akt pathway respectively. One-Way ANOVA with Bonferroni tests was applied, with significance accepted at P<0.05. Our results highlight critical, overlooked functions for Notch-3 in regulating angiogenesis and proliferation of bladder cancer cells and suggest that Notch-3 inhibitor may be drug target in bladder cancer. Further investigation into the precise mechanism for this protection is warranted.

Keyword: Bladder cancer; Noch-3; PI3K/Akt pathway; Angiogenesis; Proliferation


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