Over expression of IL-32β- exaggerated myocardial injury after ischemia and reperfusion in mice model


 

Abstract

Interleukin-32 (IL-32) is discovered as proinflammatory cytokine by inducing IL-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α. However, there are unclear data regarding IL-32β associated with worsening of myocardial injury after cardiac ischemia and reperfusion (I/R). In this study, we investigate the prognostic value of IL-32β in inflammatory response after myocardial injury. Anesthetized mice subjected to the myocardial ischemia for 30 min and 2 hours reperfusion. Expression and Regulation of IL-32β were measured by RNA Isolation and Real-Time Polymerase Chain Reaction, inflammatory response in blood and myocardial tissue, were assayed accordingly by ELISA and Western blotting, while Echo for cardiac elements measurement. The I/R group had a significantly higher expression level of IL-32β (0.643±0.012, vs. sham group 0.121±0.013; P<0.05) and associated with worsen myocardial injury, and low cardiac function. In-conclusion, IL-32β might be a new marker associated with adverse event after myocardial injury and may contribute with cardiac remodeling.

Keywords: IL-32β; Polymerase Chain Reaction; Ischemia and Reperfusion

Copyright © 2018 by The American Society for BioMedicine and BM-Publisher, Inc.

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Research Article
DOI: http://dx.doi.org/10.18081/2333-5106/019-02/57-69
American Journal of BioMedicine Volume 7, Issue 2, pages 57-69
Received October 30, 2018; accepted January 12, 2019; published February 04, 2019

How to cite this article
Jonathan A. Serody, Kerry R. Russell, Rui Chen, Mark B. Ferrara. Over expression of IL-32β- exaggerated myocardial injury after ischemia and reperfusion in mice model. American Journal of BioMedicine 2019;7(2):57-69.

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