Research Article
American Journal of BioMedicine
Volume 12, Issue 2, 2024, Pages 59-66 10.18081/2333-5106/2024.12/59
Ozge Doğan, Şermin Linton , Ceren Öztürk , Oruç Murat 1 *
Received 30 December 2023; revised 11 April 2024; accepted 20 April 2024; published 17 May 2024
Abstract
Breast cancer is the most frequently diagnosed malignancy among women worldwide. The occurrence rate of metastasis is also increased. It’s reported that 20–30% of breast cancer patients may develop metastases after diagnosis and primary tumor treatment, and approximately 90% of cancer-related deaths are attributed to metastasis. Recently, a growing number of studies have highlighted the significant role of miR-574-5p, a member of the miRNA family, in multiple human diseases that induce apoptosis in cancer cell lines completely understood yet. the majority of studies indicated that miR-574-5p was a promoter of NSCLC development. indicated that miR-574-5p was involved in the progression and metastasis of NSCLC. The purpose of this study is to investigate the effect of miR-574-5p migration in breast cancer. Seventy tumor and adjacent non-tumor tissues were examined in the study. The effects on cell proliferation and migration were investigated with MTT assay and scratch test, respectively. The effects on cell proliferation and migration were investigated with MTT assay and scratch test, respectively. Bioinformatics analysis was performed through enrichment and hub gene finding for miRNA targets. Metastatic breast cancer, proliferation, and migration-related to overexpression of miR-574-5p. In conclusion; miR-574-5p increased in breast cancer cell lines with a role in the growth, metastasis, and migration of breast cancer.
Keywords: miR-574-5p; Breast cancer; Proliferation
Copyright © 2024 Murat, et al. This article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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1. Mohammed H, Russell IA, Stark R, et al. Progesterone receptor modulates ERα action in breast cancer. Nature. 2015;523(7560):313-317. https://doi.org/10.1038/nature14583 |
|||
2. Wu SG, Wang J, Lei J, et al. Prognostic validation and therapeutic decision-making of the AJCC eighth pathological prognostic staging for T3N0 breast cancer after mastectomy. Clinical and Translational Medicine. 2020;10(1):125-136. https://doi.org/10.1002/ctm2.3 |
|||
3. Lange CA, Richer JK, Horwitz KB. Hypothesis: progesterone primes breast cancer cells for cross-talk with proliferative or antiproliferative signals. Mol Endocrinol. 1999;13(6):829-836. https://doi.org/10.1210/mend.13.6.0290 |
|||
4. Goodman ML, Trinca GM, Walter KR, et al. Progesterone receptor attenuates STAT1-mediated IFN signaling in breast cancer. J Immunol. 2019;202(10):3076-3086. https://doi.org/10.4049/jimmunol.1801152 |
|||
5. Graham JD, Yeates C, Balleine RL, et al. Characterization of progesterone receptor A and B expression in human breast cancer. Cancer Res. 1995;55(21):5063-5068. | |||
6. Cserni G, Amendoeira I, Apostolikas N, et al. Pathological work-up of sentinel lymph nodes in breast cancer. Review of current data to be considered for the formulation of guidelines. European Journal of Cancer. 2003;39(12):1654-1667. https://doi.org/10.1016/S0959-8049(03)00203-X |
|||
7. Elston CW, Ellis IO, Pinder SE. Pathological prognostic factors in breast cancer. Critical Reviews in Oncology/Hematology . 1999;31(3):209-223. https://doi.org/10.1016/S1040-8428(99)00034-7 |
|||
8. Cork DM, Lennard TW, Tyson-Capper AJ. Alternative splicing and the progesterone receptor in breast cancer. Breast Cancer Res. 2008;10(3):207. https://doi.org/10.1186/bcr2097 |
|||
9. Jacobsen BM, Schittone SA, Richer JK, Horwitz KB. Progesterone-independent effects of human progesterone receptors (PRs) in estrogen receptor-positive breast cancer: PR isoform-specific gene regulation and tumor biology. Mol Endocrinol. 2005;19(3):574-587. https://doi.org/10.1210/me.2004-0287 |
|||
10. Mote PA, Bartow S, Tran N, Clarke CL. Loss of co-ordinate expression of progesterone receptors A and B is an early event in breast carcinogenesis. Breast Cancer Res Treat. 2002;72(2):163-172. https://doi.org/10.1023/A:1014820500738 |
|||
11. Recouvreux MS, Diaz Bessone MI, Taruselli A, et al. Alterations in progesterone receptor isoform balance in normal and neoplastic breast cells modulates the stem cell population. Cells. 2020;9(9):2074. https://doi.org/10.3390/cells9092074 |
|||
12. Bonneterre J, Bosq J, Jamme P, et al. Tumour and cellular distribution of activated forms of PR in breast cancers: a novel immunohistochemical analysis of a large clinical cohort. ESMO Open. 2016;1(4):e000072. https://doi.org/10.1136/esmoopen-2016-000072 |
|||
13. Iaquinta MR, Lanzillotti C, Mazziotta C, et al. The role of microRNAs in the osteogenic and chondrogenic differentiation of mesenchymal stem cells and bone pathologies. Theranostics. 2021;11:6573-6591. https://doi.org/10.7150/thno.55664 |
|||
14. Pedroza DA, Subramani R, Lakshmanaswamy R. Classical and non-classical progesterone signaling in breast cancers. Cancers. 2020;12(9):2440. https://doi.org/10.3390/cancers12092440 |
|||
15. Poulard C, Treilleux I, Lavergne E, et al. Activation of rapid oestrogen signalling in aggressive human breast cancers. EMBO Mol Med. 2012;4(11):1200-1213. https://doi.org/10.1002/emmm.201201615 |
|||
16. Saliminejad K, Khorram Khorshid H, Soleymani Fard S, Ghaffari S. An overview of microRNAs: Biology, functions, therapeutics, and analysis methods. J. Cell. Physiol. 2019;234:5451-5465. https://doi.org/10.1002/jcp.27486 |
|||
17. Bautista-Sánchez D, Arriaga-Canon C, Pedroza-Torres A, et al. The Promising Role of miR-21 as a Cancer Biomarker and Its Importance in RNA-Based Therapeutics. Mol. Ther. Nucleic Acids. 2020;20:409-420. https://doi.org/10.1016/j.omtn.2020.03.003 |
|||
18. Ha M, Kim V. Regulation of microRNA biogenesis. Nat. Rev. Mol. Cell Biol. 2014;15:509-524. https://doi.org/10.1038/nrm3838 |
|||
19. Wang H, Meng K, Chen W, Feng D, Jia Y, Xie L. Serum miR-574-5p: A prognostic predictor of sepsis patients. Shock. 2012;37:263-267. https://doi.org/10.1097/SHK.0b013e318241baf8 |
|||
20. Liu S, Zhao L, Zhang L, Qiao L, Gao S. Downregulation of miR-574-5p inhibits HK-2 cell viability and predicts the onset of acute kidney injury in sepsis patients. Ren. Fail. 2021;43:942-948. https://doi.org/10.1080/0886022X.2021.1939051 |
|||
21. Li J, Tiwari A, Mirzakhani H, et al. Circulating MicroRNA: Incident Asthma Prediction and Vitamin D Effect Modification. J. Pers. Med. 2021;11:307. https://doi.org/10.3390/jpm11040307 |
|||
22. Zheng D, Ding Y, Ma Q, et al. Identification of Serum MicroRNAs as Novel Biomarkers in Esophageal Squamous Cell Carcinoma Using Feature Selection Algorithms. Front. Oncol. 2018;8:674. https://doi.org/10.3389/fonc.2018.00674 |
|||
23. Boileau A, Cardenas CLL, Courtois A. MiR-574-5p: A Circulating Marker of Thoracic Aortic Aneurysm. Int. J. Mol. Sci. 2019;20:3924. https://doi.org/10.3390/ijms20163924 |
|||
24. Jones JA, Stroud RE, O'Quinn EC, et al. Selective microRNA suppression in human thoracic aneurysms: Relationship of miR-29a to aortic size and proteolytic induction. Circ. Cardiovasc. Genet. 2011;4:605-613. https://doi.org/10.1161/CIRCGENETICS.111.960419 |
|||
25. Merk DR, Chin JT, Dake BA, et al. miR-29b participates in early aneurysm development in Marfan syndrome. Circ. Res. 2012;110:312-324. https://doi.org/10.1161/CIRCRESAHA.111.253740 |
|||
26. Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat. Cell Biol. 2007;9:654-659. https://doi.org/10.1038/ncb1596 |
|||
27. Hergenreider E, Heydt S, Treguer K, et al. Atheroprotective communication between endothelial cells and smooth muscle cells through miRNAs. Nat. Cell Biol. 2012;14:249-256. https://doi.org/10.1038/ncb2441 |
|||
28. Zhou J, Shao G, Chen X, et al. miRNA 206 and miRNA 574-5p are highly expression in coronary artery disease. Biosci. Rep. 2015;36:e00295. https://doi.org/10.1042/BSR20150206 |
.
How to cite
Doğan O, Linton S, Öztürk C, Murat O. MiR-574-5p dysregulation in metastatic breast cancer through cell migration. American Journal of BioMedicine 2024; 12(2):59-66.
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