Effects of caffeic acid on doxorubicin induced cardiotoxicity in rats

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Doxorubicin (Dox) is one of the most potent broad-spectrum antitumor anthracycline antibiotics, its use is limited by the development of life-threatening cardiomyopathy. Doxorubicin generates free radicals and induces oxidative stress associated with cellular injury. Further, it has been shown that free radicals are involved in doxorubicin-induced toxicity. The goal of this study is to investigated the cardio-protective effects of caffeic acid on doxorubicin induced cardiotoxicity. The rats were randomized into three equal groups, sham group without treatment, doxorubicin treated group at a dose 3mg/kg IP every other two days and group treated with doxorubicin plus caffeic acid 40mg/day. Two weeks later LV function measurment were performed and blood samples were collected from the heart to measurment plasma levels of cardiac Troponin-I (cTn-I), oxidative stress parameter malondialdehyde (MDA) and high a sensitive c-reactive protein (hs-CRP). The hearts were excised for cardiac tissue cytokines (TNF-α, IL-1β, IL-10) measurement and microscopic examination. Rats in the Dox+caffeic acid group had improved LV function, reduced cytokine expression, decreased myocardial marker injury (cTn-I) and less MDA, hs-CRP levels in comparison with the Dox group. Pathological finding appeared nearly normal in the Dox+caffeic acid without fibrosis. The results of the present study reveal that caffeic acid has a promising cardioprotective effect against doxorubicin-induced cardiotoxicity.

Keywords: Doxorubicin; Cardiotoxicity; Inflammatory response; Left ventricular function

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Research Article
DOI: http://dx.doi.org/10.18081/ajbm/2333-5106-013-12/23-27
American Journal of BioMedicine Volume 2, Issue 3, pages 14-26
Received 02 September 2013; accepted 17 January 2014; published January 28, 2014

How to cite this article
Mohammad BI, Aharis NA, Yousif MG, Alkefae Z, Hadi N. Effects of caffeic acid on doxorubicin induced cardiotoxicity in rats. American Journal of BioMedicine 2014;2(1):14-26.

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3. Introduction
4. Methods
5. Results
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