Advertisement

Critical role of TLR-4/NF-κB pathway in upregulation mechanism of cerebral ischemia reperfusion injury of mouse

 
crossMark
 
Research Article 
dx.doi.org/10.18081/2333-5106/016-4/91-102
American Journal of BioMedicine Volume 4, Issue 4, pages 91-102
Received December 11, 2015; accepted March 02, 2016; published April 26, 2016

Jonathan M. Florentina, Yun Linb, Jasdeep Singha, Le Luo Guanamail of corresponding author

Abstract

The objective of this study is to investigate the expression of TLR-4/ NF-κB pathway in the cerebral ischemia injury of male adult mice. TLR-4 mutant (HeJ) mice and TLR-4 wild (HeN) mice were divided into the stroke model group and sham group. The stroke model was made by transient cerebral ischemia through middle cerebral artery occlusion (MCAO), while mice in the sham group were manipulated in the same way, but the MCA was not occluded. The brain tissues were collected for the morphological observation. The immunohistochemistry was employed to detect the expression of NF-κB, Western blot was used to detect the expression of TLR-4 and ELISA to detect the expression of serum pro-inflammatory mediators. The expression of NF-κB in HeJ mice after the cerebral I/R was significantly lower than that in HeN mice. For the stroke model group and sham group, the expression of TLR-4 in HeN mice was all significantly higher than that in HeJ mice, while the expression of TLR-4 in HeJ mice in the stroke model group was significantly higher than that in sham group. The expression of all pro-inflammatory mediators with HeJ was significantly lower than that in HeN mice. Together, these data concluded that TLR-4/NF-κB pathway is closely related to the cerebral I/R injury, which plays its role through the release of pro-inflammatory cytokines..

KeyWords: TLR-4; MCAO; NF-κB; HeN; HeJ

Copyright © 2016 by The American Society for BioMedicine and BM-Publisher, Inc.

Article citationReferencesFull-Text/PDFFeedback
The citation data is computed by the following citation measuring services: 

Google scholarcitedby

References

1. Weeks A. The prevention and treatment of postpartum hemorrhage: what do we know, and where do we go to next? BJOG 2015;122: 202-210. [Abstract/Full-Text]

2. Gibbs Ronald. Danforth’s obstetrics and gynecology 10th edition Philadelphia Lippincott William Wilkins. p 453, 2008.

3. Xerri Y, Evans MN, Inoue G, Hanley TK, Hainz DL. Taurine attenuates inflammatory response following cerebral ischemia and reperfusion. American Journal of BioMedicine 2014;2
(5):542–554.
[Abstract/Full-Text]

4. Ministry of Health, NSW. Maternity – Prevention, Early Recognition & Management of Postpartum Haemorrhage (PPH). NSW Kids & Families 9391 9503, 2010. [Full-Text]

5. GBD 2013 Mortality and Causes of Death Collaborators. Global, regional, and national age–sex specific all-cause and cause-specific mortality for 240 causes of death, 1990–2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet 2015;385(9963):117–171. [Abstract/Full-Text]

6. Anderson JM btches D. Prevention and management of postpartum hemorrhage. American family physician 2011;75.

7. Belfort MA. Overview of postpartum hemorrhage. Wolters Kluwer 2016. [Link]

8. Sullivan E, Hall B, King J. Maternal deaths in Australia 2003-2005 Australian Institute of Health and Welfare. Australian Institute of Health and Welfare Canberra 2008. [Link PDF]

9. United Nations. The Millennium Development Goals Report 2008. New York: United Nations; 2008. [Link PDF]

10. World Health Organization (WHO). MPS Technical Update: Prevention of Postpartum Haemorrhage by Active Management of the Third Stage of Labour. Geneva: WHO; 2006. [Link PDF]

11. Haynes K, Stone C, King J. Major morbidities associated with childbirth in Victoria: Obstetric haemorrhage and associated hysterectomy Public Health Group. Department of Human Services, Melbourne; 2004. [Link PDF]

12. Knight M, Callaghan WM, Berg C, et al. Trends in postpartum haemorrhage in high resource countries: a review and recommendations from International Postpartum Haemorrhage Collaborative Group. BMC Pregnancy & Childbirth 2008;9:55. [PubMed]

13. WHO recommendation for the prevention and treatment of postpartum hemorrhage. Geneva; 2012. [Link PDF]

14. Francois K. Grand Rounds: Managing uterine atony and hemorrhagic shock Critical care in Obstetrics; 2008. [Link PDF]

15. Management of the third stage of labour to prevent post-partum haemorrhage. Journal of Midwifery & Women’s Health 2004;49(1);76-77.

16. Biguzzi E. Franchi F. Ambrogi F, et al. Risk factors for postpartum hemorrhage in a cohort of 6011 Italian women. Thrombosis Research 2012;129(4):e1-7. [PubMed]

17. Jaleel R, Khan A. Post-partum haemorrhage–a risk factor analysis. Mymensingh Med J 2010;19(2):282-9. [PubMed]

18. Leduc D, Ottawa ON, Biringer A, et al. Induction of Labour. J Obstet Gynaecol Can 2013;35(9):840-60. [PubMed]

19. Magann EF, Evans S, Hutchinson M, Collins R, Lanneau G, Morrison JC. Postpartum hemorrhage after cesarean delivery: an analysis of risk factors. South Med J 2005;98(7):681-5. [PubMed]

20. Conde-Agudelo A, Belizán JM, Lammers C. Maternal-perinatal morbidity and mortality associated with adolescent pregnancy. AJOG 2005;192:342-349. [Abstract/Full-Text]

21. Sheiner E, Sarid L, Levy A, Seidman DS, Hallak M. Obstetric risk factors and outcome of pregnancies complicated with early postpartum hemorrhage: A population-based study. J Matern Fetal Neonatal Med 2005;18(3):149-54. [PubMed]

22. Başer E, Seçkin KD, Erkılınç S, et al.The impact of parity on perinatal outcomes in pregnancies complicated by advanced maternal age. J Turk Ger Gynecol Assoc 2013;14(4):205-209. [PubMed]

23. Lao TT, Sahota DS, Cheng YK, Law LW, Leung TY. Advanced maternal age and postpartum hemorrhage – risk factor or red herring. J Matern Fetal Neonatal Med 2014;27(3):243-6. [PubMed]

24. Oyelese Y, Ananth CV. Postpartum Hemorrhage: Epidemiology, Risk Factors, and Causes. Clin Obstet Gynecol 2010;53(1):147-56. [PubMed]

25. Edhi MM,Aslam, HM Naqvi Z, Hashmi H. Postpartum hemorrhage: causes and management. BMC Res Notes 2013;6:236. [Abstract/Full-Text]

26. Parnas M, Sheiner E, Shoham-Vard I, et al. Moderate to severe thrombocytopenia during pregnancy. European Journal of Obstetrics & Gynecology and Reproductive Biology 2005;12;1-6.[Abstract/Full-Text]

27. Kramer MS, Berg C, Abenhaim H, et al. Incidence, risk factors, and temporal trends in severe postpartum hemorrhage. Am J Obstet Gynecol 2013;209(5):449.e1-7. [PubMed]

28. Callaghan WM, Kuklina EV, Berg CJ. Trends in postpartum hemorrhage: United States. 1994-2006. AJOG 2010;202(4); 353.e1-353.e6. [Abstract/Full-Text]

29. Alexander S, Dodds L, Armson BA. Perinatal Outcomes in Women With Asthma During Pregnancy. Obstet Gynecol 1998;92(3):435-40. [PubMed]

30. Liu S, Wen SW, Demissie K, Marcoux S, Kramer MS. Maternal asthma and pregnancy outcomes: A retrospective cohort study. Am J Obstet Gynecol 2001;184(2):90-6. [PubMed]   

31. Yasmeen S, Wilkins EE, Field NT, Sheikh RA, Gilbert WM. Pregnancy outcomes in women with systemic lupus erythematosus. J Matern Fetal Med 2001;10(2):91-6. [PubMed]   

32. El-Refaey E, Rodeck C. Post-partum haemorrhage: definitions, medical and surgical management. A time for change. Br Med Bull 2003;67(1):205-217. [Abstract/Full-Text]

33. Weisbrod AB, Sheppard FR, Chernofsky MR, et al. Emergent management of postpartum hemorrhage for the general and acute care surgeon. World J Emerg Surg 2009;25;4:43.  [PubMed]

34. Charbit B, Mandelbrot L, Samain E, et al. The decrease of fibrinogen is an early predictor of the severity of postpartum hemorrhage. J Thromb Haemost 2007;5(2):266-73.  [PubMed]

35. Holm C, Langhoff-Roos J, Petersen KB, Norgaard A, Diness BR. Severe postpartum haemorrhage and mode of delivery: a retrospective cohort study. BJOG 2012;119(5):596-604.  [PubMed]

36. Liu S, Liston RM, Joseph KS, et al. Maternal mortality and severe morbidity associated with low-risk planned cesarean delivery versus planned vaginal delivery at term. CMAJ 2007;13;176(4):455-460. [PubMed]

READ THE FULL ARTICLE

limited access

1. Access this article  through your login credentials or your institution

Get Access

2. Access this article  through OpenAthens/Sign in

3. Purchase this article at rate $55.00 and received Full-Text/PDF
You will have online immediate access to article following the completion of this purchase and you may download and print a copy of each article for your personal use. Use the coding below to purchase your article as PDF by credit card, debit card, payball will be asked to supply your billing card information. Before continue with your purchase please read carefully the AJBM terms and conditions of purchase.

Purchase Article

 For any technique error please contact us and will be response to sending purchase article by email.

Thank you for visiting American Journal of BioMedicine.  * = Required fields

[gravityform id=”6″ name=”Feedback”]

American Journal of Biomedicine © 2017 Frontier Theme
%d bloggers like this: